Overview

  • Product nameAnti-p15 INK4b antibody
    See all p15 INK4b primary antibodies
  • Description
    Rabbit polyclonal to p15 INK4b
  • SpecificityDetects endogenous levels of total p15 INK protein
  • Tested applicationsSuitable for: IP, WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from human p15 INK

  • Positive control
    • HeLa cell extract

Properties

Applications

Our Abpromise guarantee covers the use of ab53034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).
ELISA 1/5000.
IHC-P Use a concentration of 2 µg/ml.

Target

  • FunctionInteracts strongly with CDK4 and CDK6. Potent inhibitor. Potential effector of TGF-beta induced cell cycle arrest.
  • Sequence similaritiesBelongs to the CDKN2 cyclin-dependent kinase inhibitor family.
    Contains 4 ANK repeats.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDK inhibitory protein antibody
    • CDK4B Inhibitor antibody
    • Cdkn2b antibody
    • CDN2B_HUMAN antibody
    • Cyclin dependent kinase 4 inhibitor B antibody
    • Cyclin Dependent Kinase Inhibitor 2B antibody
    • Cyclin dependent kinase inhibitor 2B p15 inhibits CDK4 antibody
    • Cyclin dependent kinases 4 and 6 binding protein antibody
    • Cyclin-dependent kinase 4 inhibitor B antibody
    • INK4B antibody
    • MTS 2 antibody
    • MTS-2 antibody
    • MTS2 antibody
    • Multiple tumor suppressor 2 antibody
    • Multiple Tumor Supressor 2 antibody
    • OTTHUMP00000021154 antibody
    • OTTHUMP00000021155 antibody
    • p14 CDK inhibitor antibody
    • p14 INK4b antibody
    • p14-INK4b antibody
    • P15 antibody
    • p15 CDK inhibitor antibody
    • p15 inhibits CDK4 antibody
    • p15 INK4b antibody
    • p15-INK4b antibody
    • p15INK4B antibody
    • TP 15 antibody
    • TP15 antibody
    see all

Anti-p15 INK4b antibody images

  • p15 INK4b was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to p15 INK4b and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).

    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.

    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab53034.

    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).

    Band: 15kDa; p15 INK4b

  • All lanes : Anti-p15 INK4b antibody (ab53034) at 1/500 dilution

    Lane 1 : HeLa cell extract
    Lane 2 : HeLa cell extract with peptide


    Predicted band size : 15 kDa
    Observed band size : 15 kDa
  • Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system (Dako PT Link), at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
  • ab53034 (2µg/ml) staining p15 INK4b in human colon carcinoma.
    Sections were stained using an automated system (DAKO Autostainer Plus ), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 . Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

References for Anti-p15 INK4b antibody (ab53034)

This product has been referenced in:
  • Xu S  et al. ANRIL lncRNA triggers efficient therapeutic efficacy by reprogramming the aberrant INK4-hub in melanoma. Cancer Lett N/A:N/A (2016). WB, IHC ; Human . Read more (PubMed: 27461581) »
  • Serra RW  et al. A KRAS-directed transcriptional silencing pathway that mediates the CpG island methylator phenotype. Elife 3:e02313 (2014). WB ; Human . Read more (PubMed: 24623306) »

See all 5 Publications for this product

Product Wall

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I could certainly offer you a new vial as a free of charge replacement. Thank you for confirming the species and the application you wish to use the antibody. It looks that we have several antibodies against p15 I...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - nuclear (Hela Cells)
Loading amount 20 µg
Specification Hela Cells
Gel Running Conditions Non-reduced Denaturing (15% gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Jun 13 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"