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Synthetic peptide within Human p15 INK4b aa 109-138 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: P42772
Our Abpromise guarantee covers the use of ab170851 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/100 - 1/500. Predicted molecular weight: 15 kDa.|
|ICC/IF||1/10 - 1/50.|
|Flow Cyt||1/10 - 1/50. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
Immunohistochemical analysis in formalin-fixed, paraffin-embedded Human lung tissue, labeling p15 INK4b using ab170851 at a 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescence analysis of Hela cells, labeling p15 INK4b using ab170851 at a 1/10 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclei (blue).
Flow cytometric analysis of 293 cells, labeling p15 INK4b using ab170851 at a 1/10 dilution (right histogram), compared to negative control cells (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ab170851 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"