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Immunocytochemistry/ Immunofluorescence - p150 CAF1 antibody [p150-1] (ab24746)
![Immunocytochemistry/ Immunofluorescence - p150 CAF1 antibody [p150-1] (ab24746)](/ps/datasheet/images/ab24746_1.jpg)
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Product Name
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p150 CAF1 antibody [p150-1]
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See all p150 CAF1 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [p150-1] to p150 CAF1
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Immunogen
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Purified human CAF-1 protein
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Reacts with
(species key)
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Hu
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Specificity
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This antibody is specific for p150, the subunit of CAF1 that directly contacts PCNA. Immunostaining with anti-CAF1 antibody localizes the protein to sites of DNA replication in the cell.
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Tested applications
(see key)
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ICC/IF, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a dilution of 1/100.
IP: 1/150.
WB: 1/1000. Predicted molecular weight: 150 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> Chromosome Structure >> Chromatin assembly
Chromatin and Nuclear Signaling >> Chromatin Remodeling >> Histone chaperones
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Relevance
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Chromatin assembly factor 1 (CAF1) is a member of the histone chaperone family. It is required for inheritance of epigenetically determined chromosomal states, and in vitro, it promotes the assembly of chromatin during DNA replication and DNA repair. CAF1 is a complex of three subunits; p150, p60, and p48. This complex is localized to the nucleus of human cells. Both the composition and the biochemical activity of CAF1 are conserved among human, yeast, and Drosophila.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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p150-1
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Isotype
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IgG1
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Purity
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Ascites
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Storage buffer
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Preservative: None
Constituents: Ascites
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Form
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Liquid
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Concentration
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5.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this p150 CAF1 antibody [p150-1] is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for p150 CAF1 antibody [p150-1] images, references, products related to ab24746 and other tools.
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p150 CAF1 antibody [p150-1] images:
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 Immunocytochemistry/ Immunofluorescence - p150 CAF1 antibody [p150-1] (ab24746)
ab24746 staining cultured human HeLa cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.5% Triton X100 prior to blocking in 5% BSA for 1 hour at 20°C. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 20°C. A Cy3® conjugated donkey anti-mouse antibody diluted 1/400 was used as the secondary. This image is courtesy of an Abreview submitted by Dr Alexander Rapp
See Abreview
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Search PubMed (MEDLINE) for references to p150 CAF1
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p150 CAF1 antibody [p150-1] - more information
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Customer reviews (feedback) regarding p150 CAF1 antibody [p150-1] |
Customer FAQs regarding p150 CAF1 antibody [p150-1] |
Protocols for p150 CAF1 antibody [p150-1] |
Price and availability of products related to p150 CAF1 antibody [p150-1] |
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p150 CAF1 antibody [p150-1] - related products:
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p150 CAF1 antibody [p150-1] - other tools:
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Contact Abcam with a Technical Enquiry about p150 CAF1 antibody [p150-1] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Immunocytochemistry/ Immunofluorescence - p150 CAF1 antibody [p150-1] (ab24746)](/index.html?datasheet=24746#ab24746_1.jpg)
