![Immunocytochemistry/ Immunofluorescence - p150 CAF1 antibody [p150-1] (ab24746) image](#ab24746_1.jpg)
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Overview
Product name
Anti-p150 CAF1 antibody [p150-1]
See all p150 CAF1 products (3) ...
Description
Mouse monoclonal [p150-1] to p150 CAF1
Specificity
This antibody is specific for p150, the subunit of CAF1 that directly contacts PCNA. Immunostaining with anti-CAF1 antibody localizes the protein to sites of DNA replication in the cell.
Tested applications
WB, IP, ICC/IFmore details
Cross reactivity
Reacts with
Human
Immunogen
Purified human CAF-1 protein
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Ascites
Concentration
Concentration information loading...
Purity
Ascites
Clonality
Monoclonal
Clone number
p150-1
Isotype
IgG1
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Immunocytochemistry/ Immunofluorescence - p150 CAF1 antibody [p150-1] (ab24746)
![Immunocytochemistry/ Immunofluorescence - p150 CAF1 antibody [p150-1] (ab24746) image](/ps/datasheet/Images/24/ab24746/ab24746_1.jpg)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab24746 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
IP
Application notes
ICC/IF: Use at a dilution of 1/100.
IP: 1/150.
WB: 1/1000. Predicted molecular weight: 150 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Chromatin assembly factor 1 (CAF1) is a member of the histone chaperone family. It is required for inheritance of epigenetically determined chromosomal states, and in vitro, it promotes the assembly of chromatin during DNA replication and DNA repair. CAF1 is a complex of three subunits; p150, p60, and p48. This complex is localized to the nucleus of human cells. Both the composition and the biochemical activity of CAF1 are conserved among human, yeast, and Drosophila.
Cellular localization
Nuclear
Alternative names
- CAF 1 150 kDa subunit antibody
- CAF 1 antibody
- CAF 1 subunit A antibody
see all
Database links
- Entrez Gene: 10036 Human
- Omim: 601246 Human
- SwissProt: Q13111 Human
- Unigene: 79018 Human
Anti-p150 CAF1 antibody [p150-1] images:
Immunocytochemistry/ Immunofluorescence - p150 CAF1 antibody [p150-1] (ab24746)
ab24746 staining cultured human HeLa cells by ICC/IF. Cells were PFA fixed and permeabilized in 0.5% Triton X100 prior to blocking in 5% BSA for 1 hour at 20°C. The primary antibody was diluted 1/100 and incubated with the sample for 1 hour at 20°C. A Cy3® conjugated donkey anti-mouse antibody diluted 1/400 was used as the secondary.
This image is courtesy of an Abreview submitted by Dr Alexander Rapp
References for Anti-p150 CAF1 antibody [p150-1] (ab24746)
ab24746 has not yet been referenced specifically in any publications.
Publishing research using ab24746? Please let us know so that we can cite the reference in this datasheet
![p150 CAF1 antibody [p150-1] for ICC/IF in Human (24746)](/ps/Reviews/Images/ab10534_54226.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"