Overview

  • Product nameAnti-p150 CAF1 antibody [1SS-48]
    See all p150 CAF1 primary antibodies
  • Description
    Mouse monoclonal [1SS-48] to p150 CAF1
  • Specificityab30327 recognises Chromatin Assembly Factor 1 (CAF-1).
  • Tested applicationsSuitable for: ICC/IF, IP, WBmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Human p150 CAF1 subunit protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab30327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
IP
WB
  • Application notesIF: Use at a concentration of 2.5 µg/ml.
    IP: Use at a concentration of 2.5 µg/sample.
    WB: Use at a concentration of 2.5 µg/ml. Predicted molecular weight: 105 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCore component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci (By similarity). The CCR4-NOT complex functions as general transcription regulation complex. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.
    • Sequence similaritiesBelongs to the CHAF1A family.
    • Developmental stageActive complex is found in G1, S and G2 phases.
    • DomainContains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationNucleus. DNA replication foci.
    • Information by UniProt
    • Database links
    • Alternative names
      • CAF 1 antibody
      • CAF 1 subunit A antibody
      • CAF antibody
      • CAF I 150 kDa subunit antibody
      • CAF I p150 antibody
      • CAF-1 subunit A antibody
      • CAF-I 150 kDa subunit antibody
      • CAF-I p150 antibody
      • CAF1 antibody
      • CAF1A_HUMAN antibody
      • CAF1B antibody
      • CAF1P150 antibody
      • CHAF1A antibody
      • Chromatin assembly factor 1 subunit A antibody
      • Chromatin Assembly Factor 1 Subunit A p150 antibody
      • Chromatin assembly factor I (150 kDa) antibody
      • Chromatin assembly factor I 150 kDa antibody
      • Chromatin assembly factor I p150 subunit antibody
      • DCAF1 antibody
      • hp150 antibody
      • MGC71229 antibody
      • P150 antibody
      see all

    References for Anti-p150 CAF1 antibody [1SS-48] (ab30327)

    ab30327 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab30327.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"