Overview

  • Product name
    Anti-p150 CAF1 antibody [1SS-48]
    See all p150 CAF1 primary antibodies
  • Description
    Mouse monoclonal [1SS-48] to p150 CAF1
  • Specificity
    ab30327 recognises Chromatin Assembly Factor 1 (CAF-1).
  • Tested applications
    Suitable for: ICC/IF, IP, WBmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Human p150 CAF1 subunit protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab30327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
IP
WB
  • Application notes
    IF: Use at a concentration of 2.5 µg/ml.
    IP: Use at a concentration of 2.5 µg/sample.
    WB: Use at a concentration of 2.5 µg/ml. Predicted molecular weight: 105 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Core component of the CAF-1 complex, a complex thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer. CHAF1A binds to histones H3 and H4. It may play a role in heterochromatin maintenance in proliferating cells by bringing newly synthesized cbx proteins to heterochromatic DNA replication foci (By similarity). The CCR4-NOT complex functions as general transcription regulation complex. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.
    • Sequence similarities
      Belongs to the CHAF1A family.
    • Developmental stage
      Active complex is found in G1, S and G2 phases.
    • Domain
      Contains one Pro-Xaa-Val-Xaa-Leu (PxVxL) motif, which is required for interaction with chromoshadow domains. This motif requires additional residues -7, -6, +4 and +5 of the central Val which contact the chromoshadow domain.
    • Post-translational
      modifications
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Nucleus. DNA replication foci.
    • Information by UniProt
    • Database links
    • Alternative names
      • CAF 1 antibody
      • CAF 1 subunit A antibody
      • CAF antibody
      • CAF I 150 kDa subunit antibody
      • CAF I p150 antibody
      • CAF-1 subunit A antibody
      • CAF-I 150 kDa subunit antibody
      • CAF-I p150 antibody
      • CAF1 antibody
      • CAF1A_HUMAN antibody
      • CAF1B antibody
      • CAF1P150 antibody
      • CHAF1A antibody
      • Chromatin assembly factor 1 subunit A antibody
      • Chromatin Assembly Factor 1 Subunit A p150 antibody
      • Chromatin assembly factor I (150 kDa) antibody
      • Chromatin assembly factor I 150 kDa antibody
      • Chromatin assembly factor I p150 subunit antibody
      • DCAF1 antibody
      • hp150 antibody
      • MGC71229 antibody
      • P150 antibody
      see all

    References

    ab30327 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab30327.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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