Overview

  • Product name
  • Description
    Rabbit polyclonal to P15RS
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Chicken, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 67-248 of Human P15RS (Q96P16).

  • Positive control
    • HCT116 whole cell lysate. This antibody gave a positive result when used in the following methanol fixed cell lines: HeLa.

Properties

Applications

Our Abpromise guarantee covers the use of ab126065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/3000. Predicted molecular weight: 36 kDa.

Target

  • Function
    Interacts with phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD. May act as a negative regulator of cyclin-D1 (CCND1) and cyclin-E (CCNE1) in the cell cycle.
  • Sequence similarities
    Belongs to the UPF0400 (RTT103) family.
    Contains 1 CID domain.
  • Information by UniProt
  • Database links
  • Alternative names
    • C77387 antibody
    • CDKN2B-related protein antibody
    • Cyclin dependent kinase 2B inhibitor related protein antibody
    • Cyclin dependent kinase inhibitor 2B related protein (p15INK4B related protein) antibody
    • Cyclin dependent kinase inhibitor 2B related protein antibody
    • Cyclin dependent kinase inhibitor related protein antibody
    • Cyclin-dependent kinase inhibitor 2B-related protein antibody
    • FLJ10656 antibody
    • HsT3101 antibody
    • Hypothetical protein FLJ10656 antibody
    • KIAA4077 antibody
    • MGC19513 antibody
    • MGC29396 antibody
    • MGC36325 antibody
    • mKIAA4077 antibody
    • p15INK4B related protein antibody
    • p15INK4B-related protein antibody
    • Putative uncharacterized protein P15RS antibody
    • Regulation of nuclear pre mRNA domain containing 1A antibody
    • Regulation of nuclear pre-mRNA domain-containing protein 1A antibody
    • RGD1307374 antibody
    • RPR1A_HUMAN antibody
    • RPRD1A antibody
    see all

Images

  • ICC/IF image of ab126065 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab126065 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-P15RS antibody (ab126065) at 1/1000 dilution + HCT116 whole cell lysate at 30 µg

    Predicted band size : 36 kDa

References

ab126065 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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