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was wondering if you can give me some discount for the product no ab51243. Thanks |
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ANSWER: |
Thank you for contacting us. While we do not currently offer a discount program specifically to XXXXXX, we do offer discountd off purchases of five products or more. Our customer service representative would be able to provide futher details at us.orders@abcam.com or by calling 1-888-772-2226. I'm also pleased to let you know that Abcam has generous loyalty schemes and promotions which seek to reward our valued customers thanking them for choosing Abcam as a supplier. Abpoints are awarded for Abreviews and more information can be found on our website. www.abcam.com/abreviews Please do not hesitate to contact us should you have further questions. |
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Hi, I am sorry; I thought I have provided the purchase order and order number in my previous message. Anyhow, the information is below. The purchase order number is PO XXXX and order number is XXXX. Thanks for your help, and I hope you have a wonderful weekend! |
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ANSWER: |
Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number XXXX. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know. I wish you the best of luck with your research. |
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Hi, I appreciate your prompt response. The samples are human cell extracts. I have not attempted other concentrations since the product sheet suggested the dilution of 1:20,000. Normally, I try to "optimize" the antibody concentration if there is no recommendations. Since this antibody had been tested and verified, I only tested the one concentration (1:10,000). I informed my supervisor of the situation and mentioned that Abcam is willing to send another lot. Please let me know if the situation is true. Thanks for all your help! |
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ANSWER: |
Thank you for contacting us. That is correct. I will process that for you as soon as you are able to send me the ooriginal order number or PO used to purchase this. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. |
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Hi, I appreciate your help and follow-up. I have attached the scanned film of the two western blots I performed. As I explained in our conversation, the p16 antibody from a different vendor gives a faint positive band, but the high background interferes with the signal. Therefore, I am interested in finding another antibody with better detection. The purchase order number is PO 51122A0093 and order number is 1009543. Please let me know if I need to provide with more information or documents. Thanks for your support! ________________________________ From: technical@abcam.com [technical@abcam.com] Sent: Tuesday, January 10, 2012 5:05 PM To: Nguyen, Loan Subject: Reply from Abcam to your enquiry regarding ab51243 [CCE3414585] [abcam: discover more] Dear Sir/Madam We have an answer to your inquiry: Thank you for contacting us. I wanted to contact you and thank you for calling us. I am sorry that ab51243 has not performed as it should have. I look forward to seeing your images and working this through with you. Please do not hesitate to contact us if you need any more advice or information. Help us improve our service. Rate your experience with us today. Your original inquiry to Abcam: This product is not working well. Best regards, Keith Keith Beadle Scientific Support Specialist Abcam Inc. www.abcam.com Abcam Customer Services and Scientific Support Team www.abcam.com/technical [CCE3414585] Discover more at abcam.com |
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ANSWER: |
Thank you for contacting us and for sending your data to me. I was hoping that you might remind me of the species that you are running the experiment with. Have you tried increasing the antibody concentration? When we spoke you had mentioned 1:10,000 over night, have you tried 1:5,000, 1:1,000 or even 1:500? I have thought that it may be that theremay be a cross reaction between theantibody and your block.Have you tried to change your block to BSA or gelatin? I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. |
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This product is not working well. |
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ANSWER: |
Thank you for contacting us. I wanted to contact you and thank you for calling us. I am sorry that ab51243 has not performed as it should have. Ilook forward to seeing your images and working this through with you. Please do not hesitate to contact us if you need any more advice or information. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Anti-p16 ARC antibody [EP1551Y] (ab51243) at 1/20000 dilution + Fetal brain lysate at 10 µg
Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution
Predicted band size : 16 kDa
Observed band size : 16 kDa
Ab51243 (1:50) staining human p16 ARC in human uterus adenocarcinoma by immunohistochemistry using paraffin embedded tissue.
Overlay histogram showing HeLa cells stained with ab51243 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51243, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
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