Anti-p16 ARC antibody [EP1551Y] (ab51243)

  Western blot - p16 ARC antibody [EP1551Y] (ab51243)

Anti-p16 ARC antibody [EP1551Y] (ab51243) at 1/20000 dilution + Fetal brain lysate at 10 µg

Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution

Predicted band size : 16 kDa
Observed band size : 16 kDa

  Immunohistochemistry (Paraffin-embedded sections) - p16 ARC antibody [EP1551Y] (ab51243)

Ab51243 (1:50) staining human p16 ARC in human uterus adenocarcinoma by immunohistochemistry using paraffin embedded tissue.

  Flow Cytometry - p16 ARC antibody [EP1551Y] (ab51243)

Overlay histogram showing HeLa cells stained with ab51243 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51243, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.