Overview

  • Product nameAnti-p16 ARC antibody [EP1551Y]
    See all p16 ARC primary antibodies
  • Description
    Rabbit monoclonal [EP1551Y] to p16 ARC
  • Tested applicationsICC/IF, IP, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human p16 ARC aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control
    • WB: Human fetal, mouse and rat brain tissue lysates and HeLa, HepG2, SKBR-3 and MCF-7 cell lysates. IHC-P: Human uterus adenocarcinoma and spleen tissues. ICC/IF: Neuro-2a cells. IP: Human fetal brain tissue lysate.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Produced using Abcam's RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone numberEP1551Y
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab51243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/100.
IP 1/20 - 1/70.
WB 1/1000 - 1/10000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See protocols (link: http://www.abcam.com/protocols/ihc-antigen-retrieval-protocol).

  • Application notesIs unsuitable for Flow Cyt.
  • Target

    • FunctionFunctions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.
    • Sequence similaritiesBelongs to the ARPC5 family.
    • Cellular localizationCytoplasm > cytoskeleton. Cell projection.
    • Information by UniProt
    • Database links
    • Alternative names
      • Actin related protein 2/3 complex subunit 5 (16 kD) antibody
      • Actin related protein 2/3 complex subunit 5 antibody
      • Actin related protein 2/3 complex, subunit 5 16kDa antibody
      • Actin-related protein 2/3 complex subunit 5 antibody
      • ARC16 antibody
      • Arp2/3 complex 16 kDa subunit antibody
      • Arp2/3 protein complex subunit p16 antibody
      • ARPC 5 antibody
      • Arpc5 antibody
      • ARPC5_HUMAN antibody
      • dJ127C7.3 antibody
      • MGC88523 antibody
      • p16 Arc antibody
      • p16-ARC antibody
      • RP1 127C7.3 antibody
      see all

    Anti-p16 ARC antibody [EP1551Y] images

    • All lanes : Anti-p16 ARC antibody [EP1551Y] (ab51243) at 1/5000 dilution (purified)

      Lane 1 : HeLa whole cell lysate
      Lane 2 : SKBR-3 whole cell lysate
      Lane 3 : MCF-7 whole cell lysate
      Lane 4 : HepG2 whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 16 kDa
      Observed band size : 16 kDa
      Blocking and dilution buffer: 5% NFDM/TBST
    • All lanes : Anti-p16 ARC antibody [EP1551Y] (ab51243) at 1/5000 dilution (purified)

      Lane 1 : Human fetal brain tissue lysate
      Lane 2 : Mouse brain tissue lysate
      Lane 3 : Rat brain tissue lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 16 kDa
      Observed band size : 16 kDa
      Blocking and dilution buffer: 5% NFDM/TBST
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue labelling p16 ARC with purified ab51243 at a dilution of 1/50. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

    • Immunocytochemistry/Immunofluorescence analysis of Neuro-2a cells labelling p16 ARC with purified ab51243 at a dilution of 1/100. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

      Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

      Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

    • ab51243 (purified) at a dilution of 1/20 immunoprecipitating p16 ARC in human fetal brain tissue lysate.

      Lane 1 (input): Human fetal brain tissue lysate (10µg)

      Lane 2 (+): ab51243 + human fetal brain tissue lysate.

      Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab51243 in human fetal brain tissue lysate.

      For western blotting, a HRP-conjugated anti-rabbit specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • All lanes : Anti-p16 ARC antibody [EP1551Y] (ab51243) at 1/2000 dilution (unpurified)

      Lane 1 : Human fetal brain tissue lysate
      Lane 2 : Mouse brain tissue lysate
      Lane 3 : Rat brain tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

      Predicted band size : 16 kDa

      Blocking buffer and concentration: 5% NFDM/TBST

      Diluting buffer and concentration: 5% NFDM/TBST

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human uterus adenocarcinoma tissue labelling p16 ARC with unpurified ab51243 at a dilution of 1/50.

    References for Anti-p16 ARC antibody [EP1551Y] (ab51243)

    This product has been referenced in:
    • Davis H  et al. Aberrant epithelial GREM1 expression initiates colonic tumorigenesis from cells outside the stem cell niche. Nat Med 21:62-70 (2015). Read more (PubMed: 25419707) »
    • Chang BS  et al. Collagen complexes increase the efficiency of iPS cells generated using fibroblasts from adult mice. J Reprod Dev 61:145-53 (2015). WB ; Mouse . Read more (PubMed: 25740096) »

    See all 5 Publications for this product

    Product Wall

    Application Western blot
    Loading amount 25 µg
    Gel Running Conditions Reduced Denaturing
    Sample Mouse Tissue lysate - whole (mouse lung)
    Specification mouse lung
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    Submitted Feb 27 2015

    Thank you for contacing us. I have placed the protocol below, please let me know if you have any further questions.



    2. Protocol
    2.1. Deparaffinization/Rehydration
    2.1.1. Heat slides in an oven at 65 °C for 1 hour. Read More

    Thank you for contacting us.

    We do have control lysates for both recommended positive controls, human fetal brain tissue (ab29467) and human uterus adenocarcinoma tissue (ab44053).

    I hope this information is helpful to you. Please...

    Read More

    Thank you for contacting us.
    We have not specifically tested fat tissue in rat, however it is guaranteed to work in rat and should give a signal as long as the tissue expresses p16.

    I hope this information is helpful to you. Please do not ...

    Read More

    Thank you for your enquiry which has been forwarded to the scientific support team.

    I can confirm that ab51243 Anti-p16 ARC antibody [EP1551Y] has already been tested in IHC-P and WB. We also have images for these applications. Therefore, I ...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (MCF7)
    Loading amount 100000 cells
    Specification MCF7
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    Submitted May 17 2012

    Thank you for contacting us. While we do not currently offer a discount program specifically to XXXXXX, we do offer discountd off purchases of five products or more. Our customer service representative would be able to provide futher details at us.o...

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    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused...

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    Thank you for contacting us. That is correct. I will process that for you as soon as you are able to send me the ooriginal order number or PO used to purchase this. I hope this information is helpful to you. Please do not hesitate to contact us if you ...

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    Thank you for contacting us and for sending your data to me. I was hoping that you might remind me of the species that you are running the experiment with. Have you tried increasing the antibody concentration? When we spoke you had mentioned 1:10,000 o...

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