p16 ARC antibody [EP1551Y] (ab51243)

Product Name 

p16 ARC antibody [EP1551Y]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [EP1551Y] to p16 ARC

Immunogen 

Synthetic peptide corresponding to residues on the N-terminus of human p16 ARC.

Reacts with 
(species key)

Hu, Ms, Rat

Tested applications 
(see key)

Flow Cyt, ICC/IF, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Flow Cyt: 1/20.
ICC/IF: 1/50 - 1/100.
IHC-P: 1/50 - 1/100. We strongly recommend that customers perform an antigen retrieval step.
IP: 1/70.
WB: 1/20000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Fetal brain lysate or human uterus adenocarcinoma tissue.

Cellular localization 

Cytoplasm; cytoskeleton. Cell projection.

Research areas 

Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microfilaments >> Actin etc >> Actin Assembly

Relevance 

The Arp2/3 protein complex has been implicated in the control of actin polymerization in cells. The human complex consists of seven subunits which include the actin related proteins Arp2 and Arp3, and five others referred to as p41-Arc, p34-Arc, p21-Arc, p20-Arc, and p16-Arc. These proteins localize in the cytoplasm of fibroblasts that lack lamellipodia, but are enriched in lamellipodia on stimulation with serum or platelet-derived growth factor. A dynamic role for this complex in the organization of the actin cytoskeleton has been suggested. p16-Arc has been found to be a globular alpha-helical subunit.

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

EP1551Y

Isotype 

IgG

Purity 

Tissue culture supernatant

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4

Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - why is this?

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

Produced under U.S. Patent No. 5,675,063.

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