Overview

  • Product nameAnti-p16 ARC antibody [EP1551Y]
    See all p16 ARC primary antibodies
  • Description
    Rabbit monoclonal [EP1551Y] to p16 ARC
  • Tested applicationsICC/IF, Flow Cyt, IP, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues on the N-terminus of human p16 ARC.

  • Positive control
    • Fetal brain lysate or human uterus adenocarcinoma tissue.
  • General notes

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab51243 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/100.
Flow Cyt 1/50.
IP 1/70.
WB 1/1000 - 1/10000. Detects a band of approximately 16 kDa (predicted molecular weight: 16 kDa).
IHC-P Use at an assay dependent concentration.

Target

  • FunctionFunctions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.
  • Sequence similaritiesBelongs to the ARPC5 family.
  • Cellular localizationCytoplasm > cytoskeleton. Cell projection.
  • Information by UniProt
  • Database links
  • Alternative names
    • Actin related protein 2/3 complex subunit 5 (16 kD) antibody
    • Actin related protein 2/3 complex subunit 5 antibody
    • Actin related protein 2/3 complex, subunit 5 16kDa antibody
    • Actin-related protein 2/3 complex subunit 5 antibody
    • ARC16 antibody
    • Arp2/3 complex 16 kDa subunit antibody
    • Arp2/3 protein complex subunit p16 antibody
    • ARPC 5 antibody
    • Arpc5 antibody
    • ARPC5_HUMAN antibody
    • dJ127C7.3 antibody
    • MGC88523 antibody
    • p16 Arc antibody
    • p16-ARC antibody
    • RP1 127C7.3 antibody
    see all

Anti-p16 ARC antibody [EP1551Y] images

  • All lanes : Anti-p16 ARC antibody [EP1551Y] (ab51243) at 1/2000 dilution

    Lane 1 : Human fetal brain
    Lane 2 : Mouse brain
    Lane 3 : Rat brain

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size : 16 kDa

    Blocking buffer and concentration: 5% NFDM/TBST

    Diluting buffer and concentration: 5% NFDM/TBST

  • Ab51243 (1:50) staining human p16 ARC in human uterus adenocarcinoma by immunohistochemistry using paraffin embedded tissue.Ab51243 (1:50) staining human p16 ARC in human uterus adenocarcinoma by immunohistochemistry using paraffin embedded tissue.
  • Overlay histogram showing HeLa cells stained with ab51243 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51243, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-p16 ARC antibody [EP1551Y] (ab51243)

This product has been referenced in:
  • Chang YF  et al. Interaction between microRNAs and actin-associated protein Arpc5 regulates translational suppression during male germ cell differentiation. Proc Natl Acad Sci U S A 109:5750-5 (2012). ICC/IF ; Mouse . Read more (PubMed: 22447776) »
  • Schmidt S  et al. Kindlin-3-mediated signaling from multiple integrin classes is required for osteoclast-mediated bone resorption. J Cell Biol 192:883-97 (2011). ICC/IF ; Mouse . Read more (PubMed: 21357746) »

See all 3 Publications for this product

Product Wall

Thank you for contacing us. I have placed the protocol below, please let me know if you have any further questions.



2. Protocol
2.1. Deparaffinization/Rehydration
2.1.1. Heat slides in an oven at 65 °C for 1 hour. Read More

Thank you for contacting us.

We do have control lysates for both recommended positive controls, human fetal brain tissue (ab29467) and human uterus adenocarcinoma tissue (ab44053).

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Thank you for contacting us.
We have not specifically tested fat tissue in rat, however it is guaranteed to work in rat and should give a signal as long as the tissue expresses p16.

I hope this information is helpful to you. Please do not ...

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Thank you for your enquiry which has been forwarded to the scientific support team.

I can confirm that ab51243 Anti-p16 ARC antibody [EP1551Y] has already been tested in IHC-P and WB. We also have images for these applications. Therefore, I ...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (MCF7)
Loading amount 100000 cells
Specification MCF7
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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Submitted May 17 2012

Thank you for contacting us. While we do not currently offer a discount program specifically to XXXXXX, we do offer discountd off purchases of five products or more. Our customer service representative would be able to provide futher details at us.o...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused...

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Thank you for contacting us and for sending your data to me. I was hoping that you might remind me of the species that you are running the experiment with. Have you tried increasing the antibody concentration? When we spoke you had mentioned 1:10,000 o...

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Thank you for contacting us. I wanted to contact you and thank you for calling us. I am sorry that ab51243 has not performed as it should have. Ilook forward to seeing your images and working this through with you. Please do not hesitate to contact us ...

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