Anti-p18 INK4c antibody (ab66748)

Overview

• Product nameAnti-p18 INK4c antibodySee all p18 INK4c primary antibodies ...
• Description
  Rabbit polyclonal to p18 INK4c
• Tested applicationsWB, Sandwich ELISA more details
• Species reactivity
  Reacts with: Rat, Human
  
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human p18 INK4c.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab71845.)

• Positive controlThis antibody gave a positive signal in the following lysates: Rat Thymus Tissue Jurkat Whole Cell - Staurosporine Treated (24hr, 500nM) HeLa Whole Cell

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Cell cycle
  • Cell cycle inhibitors
  • Ink4

  • Cancer
  • Oncoproteins/suppressors
  • Tumor suppressors
  • INK4a/Arf

  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Cell Cycle Inhibitors
  • Ink4

  • Cell Biology
  • Cell Cycle
  • Cell Cycle Inhibitors
  • Ink4

Applications

Target

• FunctionInteracts strongly with CDK6, weakly with CDK4. Inhibits cell growth and proliferation with a correlated dependence on endogenous retinoblastoma protein RB.
• Tissue specificityHighest levels found in skeletal muscle. Also found in pancreas and heart.
• Sequence similaritiesBelongs to the CDKN2 cyclin-dependent kinase inhibitor family.
  Contains 4 ANK repeats.

Target information above from: UniProt accession P42773 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 1031 Human
  • Entrez Gene: 29658 Rat
  • Omim: 603369 Human
  • SwissProt: P42773 Human
  • Unigene: 525324 Human
  • Unigene: 728783 Human
  • Unigene: 63865 Rat

• Alternative names
  CDK6 inhibitor p18 antibodyCDKN 2C antibodyCDKN 6 antibody

  CDKN2C antibodyCDKN6 antibodyCDN2C_HUMAN antibodyCyclin dependent inhibitor antibodyCyclin dependent kinase 4 inhibitor C antibodyCyclin dependent kinase 6 inhibitor antibodyCyclin dependent kinase 6 inhibitor p18 antibodyCyclin dependent kinase inhibitor 2C (p18 inhibits CDK4) antibodyCyclin dependent kinase inhibitor 2C antibodyCyclin-dependent kinase 4 inhibitor C antibodyCyclin-dependent kinase 6 inhibitor antibodyINK4C antibodyOTTHUMP00000046546 antibodyp18 antibodyp18 inhibits CDK4 antibodyp18 INK4c antibodyp18 INK6 antibodyp18-INK4c antibodyp18-INK6 antibody

  see all

Anti-p18 INK4c antibody images

• 

  Sandwich ELISA - p18 INK4c antibody (ab66748)
  Standard Curve for p18 INK4c (Analyte: p18 INK4c protein (T7 Tag) (ab40127)); dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [18P118 (same as DCS-118)] to p18 INK4c (ab3216) at 5ug/ml and Detector Antibody Rabbit polyclonal to p18 INK4c (ab66748) at 0.5ug/ml.
• 

  Western blot - p18 INK4c antibody (ab66748)
  All lanes : Anti-p18 INK4c antibody (ab66748) at 1 µg/ml
  
  Lane 1 : Rat Thymus Tissue Lysate
  Lane 2 : Jurkat Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
  Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size : 18 kDa
  Observed band size : 18 kDa
  Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 20 minutes

Protocols

Protocol Booklet