Overview

  • Product name
  • Description
    Rabbit polyclonal to p18 INK4c
  • Tested applications
    Suitable for: WB, Sandwich ELISAmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 100 to the C-terminus of Human p18 INK4c.

    (Peptide available as ab71845.)

  • Positive control
    • This antibody gave a positive signal in the following lysates: Rat Thymus Tissue Jurkat Whole Cell - Staurosporine Treated (24hr, 500nM) HeLa Whole Cell

Properties

Applications

Our Abpromise guarantee covers the use of ab66748 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 18 kDa).
Sandwich ELISA Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [18P118 (same as DCS-118)] to p18 INK4c (ab3216) as Capture.

Target

  • Function
    Interacts strongly with CDK6, weakly with CDK4. Inhibits cell growth and proliferation with a correlated dependence on endogenous retinoblastoma protein RB.
  • Tissue specificity
    Highest levels found in skeletal muscle. Also found in pancreas and heart.
  • Sequence similarities
    Belongs to the CDKN2 cyclin-dependent kinase inhibitor family.
    Contains 4 ANK repeats.
  • Information by UniProt
  • Database links
  • Alternative names
    • CDK6 inhibitor p18 antibody
    • CDKN 2C antibody
    • CDKN 6 antibody
    • Cdkn2c antibody
    • CDKN6 antibody
    • CDN2C_HUMAN antibody
    • Cyclin dependent inhibitor antibody
    • Cyclin dependent kinase 4 inhibitor C antibody
    • Cyclin dependent kinase 6 inhibitor antibody
    • Cyclin dependent kinase 6 inhibitor p18 antibody
    • Cyclin dependent kinase inhibitor 2C (p18 inhibits CDK4) antibody
    • Cyclin dependent kinase inhibitor 2C antibody
    • Cyclin-dependent kinase 4 inhibitor C antibody
    • Cyclin-dependent kinase 6 inhibitor antibody
    • INK4C antibody
    • OTTHUMP00000046546 antibody
    • p18 antibody
    • p18 inhibits CDK4 antibody
    • p18 INK4c antibody
    • p18 INK6 antibody
    • p18-INK4c antibody
    • p18-INK6 antibody
    see all

Images

  • Standard Curve for p18 INK4c (Analyte: p18 INK4c protein (T7 Tag) (ab40127)); dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [18P118 (same as DCS-118)] to p18 INK4c (ab3216) at 5ug/ml and Detector Antibody Rabbit polyclonal to p18 INK4c (ab66748) at 0.5ug/ml.
  • All lanes : Anti-p18 INK4c antibody (ab66748) at 1 µg/ml

    Lane 1 : Rat Thymus Tissue Lysate
    Lane 2 : Jurkat Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 18 kDa
    Observed band size : 18 kDa
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

References

ab66748 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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