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Products:Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Ink4
Overview
Product name
Anti-p19 INK4d antibody
See all p19 INK4d products (5) ...
Description
Mouse polyclonal to p19 INK4d
Tested applications
Cross reactivity
Reacts with Xiphophorus maculatus.
Immunogen
Vector coding for a partial recombinant fusion protein, corresponding to amino acids 13-112 of p19 INK4d (Xiphophorus maculatus) [Swiss Prot Q6DKY1]. Target sequence used to make the antibody: TAAAAKGNAD EVQRILEECR VHPDTPNEFG RTALQVMMMG NSKVARLLLE KGAEPNVQDK HGIAPVHDAA QTGFLETLQV LVEHGASVNI QDQNGALPIH,
TAAAAKGNAD EVQRILEECR VHPDTPNEFG RTALQVMMMG NSKVARLLLE KGAEPNVQDK HGIAPVHDAA QTGFLETLQV LVEHGASVNI QDQNGALPIH
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% Glycerol, Whole serum
Purity
Whole antiserum
Primary antibody notes
This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al.PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> INK4a/Arf
Epigenetics and Nuclear Signaling >> Cell cycle >> Cell Cycle Inhibitors >> Ink4
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Ink4
Applications
Show applications key
Our Abpromise guarantee covers the use of ab52735 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Application notes
WB: 1/1000. Predicted molecular weight: 18 kDa.
This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
Cyclins are important in regulating the cell cycle through their formation of enzymatic complexes with various cyclin dependent kinases. The activity of the cyclin D dependent kinases is controlled by inhibitors such as the INK4 family, which includes INK4a, INK4b, INK4c, and INK4d (p19 INK4d). p19 INK4d is a specific inhibitor of cdk 4 / cdk 6 and is periodically expressed during cell cycle. It is maximally induced as cells enter S phase.
Cellular localization
Cytoplasmic and Nuclear
Alternative names
- CDK inhibitor p19INK4d antibody
- CDKN 2D antibody
- CDKN2D antibody
see all
References for Anti-p19 INK4d antibody (ab52735)
ab52735 has not yet been referenced specifically in any publications.
Publishing research using ab52735? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"