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Anti-p19 INK4d antibody (ab77223) at 5 µg/ml + immunogen at 0.2 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
developed using the ECL technique
Predicted band size : 17 kDa
Observed band size : 46 kDa (why is the actual band size different from the predicted?)
The observed band size may not correspond to the predicted protein molecular weight as the immunogen was used for the sample.
All lanes : Anti-p19 INK4d antibody (ab77223) at 5 µg/ml
Lane 1 : p19 INK4d-transfected 293T cell lysate
Lane 2 : non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
developed using the ECL technique
Predicted band size : 17 kDa
Observed band size : 17 kDa
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