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Products:Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Ink4
Overview
Product name
Anti-p19 INK4d antibody
See all p19 INK4d products (5) ...
Description
Mouse monoclonal to p19 INK4d
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein (Human) with a 26kDa tag
Positive control
p19 INK4D transfected 293T cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Isotype
IgG2b
Light chain type
kappa
Research areas
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> INK4a/Arf
Epigenetics and Nuclear Signaling >> Cell cycle >> Cell Cycle Inhibitors >> Ink4
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Ink4
Applications
Show applications key
Our Abpromise guarantee covers the use of ab77223 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml.Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
ELISA: Use at an assay dependent dilution.
Target
Relevance
Cyclins are important in regulating the cell cycle through their formation of enzymatic complexes with various cyclin dependent kinases. The activity of the cyclin D dependent kinases is controlled by inhibitors such as the INK4 family, which includes INK4a, INK4b, INK4c, and INK4d (p19 INK4d). p19 INK4d is a specific inhibitor of cdk 4 / cdk 6 and is periodically expressed during cell cycle. It is maximally induced as cells enter S phase.
Cellular localization
Cytoplasmic and Nuclear
Alternative names
- CDK inhibitor p19INK4d antibody
- CDKN2D antibody
- cell cycle inhibitor, Nur77 associating protein antibody
see all
Database links
- Entrez Gene: 1032 Human
- Omim: 600927 Human
- SwissProt: P55273 Human
- Unigene: 435051 Human
Anti-p19 INK4d antibody images:
Western blot - p19 INK4d antibody (ab77223)
Anti-p19 INK4d antibody (ab77223) at 5 µg/ml + immunogen at 0.2 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
developed using the ECL technique
Predicted band size : 17 kDa
Observed band size : 46 kDa (why is the actual band size different from the predicted?)
The observed band size may not correspond to the predicted protein molecular weight as the immunogen was used for the sample.
Western blot - p19 INK4d antibody (ab77223)
All lanes : Anti-p19 INK4d antibody (ab77223) at 5 µg/ml
Lane 1 : p19 INK4d-transfected 293T cell lysate
Lane 2 : non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
developed using the ECL technique
Predicted band size : 17 kDa
Observed band size : 17 kDa
References for Anti-p19 INK4d antibody (ab77223)
ab77223 has not yet been referenced specifically in any publications.
Publishing research using ab77223? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"