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Western blot - p19 INK4d antibody (ab77223)
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Western blot - p19 INK4d antibody (ab77223)
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Product Name
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p19 INK4d antibody
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See all p19 INK4d antibodies (3)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal to p19 INK4d
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Immunogen
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Recombinant full length protein (Human) with a 26kDa tag
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Reacts with
(species key)
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Hu
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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p19 INK4D transfected 293T cell lysate
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Research areas
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Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> INK4a/Arf
Chromatin and Nuclear Signaling >> Cell cycle >> Cell Cycle Inhibitors >> Ink4
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Ink4
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Relevance
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Cyclins are important in regulating the cell cycle through their formation of enzymatic complexes with various cyclindependent kinases. The activity of the cyclin D dependent kinases is controlled by inhibitors such as the INK4 family, which includes INK4a, INK4b, INK4c, and INK4d. The p19 protein, also known as INK4d was first identified in a yeast two-hybrid system screened for CDK4 binding proteins(Hirai H, et al. Molec. Cell. Biol. 15: 2672-2681, 1995). Human p19 shares 86% identity at the amino acid level with murine p19, and is approximately 44% identical to each of the other human INK4 family members (Okuda, T. et al. Genomics 29: 623-630, 1995). p19 INK4d is a specific inhibitor of cdk 4 / cdk 6 and is periodically expressed during cell cycle. It is maximally induced as cells enter S phase.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Isotype
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IgG2b
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Light chain type
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kappa
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: PBS, pH 7.2
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this p19 INK4d antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for p19 INK4d antibody images, references, products related to ab77223 and other tools.
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p19 INK4d antibody images:
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 Western blot - p19 INK4d antibody (ab77223)
p19 INK4d antibody (ab77223) at 5 µg/ml + immunogen at 0.2 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
developed using the ECL technique
Predicted band size : 17 kDa
Observed band size : 46 kDa (why is the actual band size different from the predicted?)
The observed band size may not correspond to the predicted protein molecular weight as the immunogen was used for the sample.
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Western blot - p19 INK4d antibody (ab77223)
All lanes : p19 INK4d antibody (ab77223) at 5 µg/ml
Lane 1 : p19 INK4d-transfected 293T cell lysate
Lane 2 : non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
developed using the ECL technique
Predicted band size : 17 kDa
Observed band size : 17 kDa
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Search PubMed (MEDLINE) for references to p19 INK4d
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p19 INK4d antibody - more information
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Customer reviews (feedback) regarding p19 INK4d antibody |
Customer FAQs regarding p19 INK4d antibody |
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p19 INK4d antibody - related products:
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p19 INK4d antibody - other tools:
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Contact Abcam with a Technical Enquiry about p19 INK4d antibody |
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