Overview

  • Product nameAnti-p19 INK4d antibody
    See all p19 INK4d primary antibodies
  • Description
    Mouse polyclonal to p19 INK4d
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with Xiphophorus maculatus.
  • Immunogen

    Vector coding for a partial recombinant fusion protein, corresponding to amino acids 13-112 of p19 INK4d (Xiphophorus maculatus) [Swiss Prot Q6DKY1]. Target sequence used to make the antibody: TAAAAKGNAD EVQRILEECR VHPDTPNEFG RTALQVMMMG NSKVARLLLE KGAEPNVQDK HGIAPVHDAA QTGFLETLQV LVEHGASVNI QDQNGALPIH,

  • General notes


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, Whole serum
  • PurityWhole antiserum
  • Primary antibody notesThis antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab52735 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 18 kDa.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

Target

  • RelevanceCyclins are important in regulating the cell cycle through their formation of enzymatic complexes with various cyclin dependent kinases. The activity of the cyclin D dependent kinases is controlled by inhibitors such as the INK4 family, which includes INK4a, INK4b, INK4c, and INK4d (p19 INK4d). p19 INK4d is a specific inhibitor of cdk 4 / cdk 6 and is periodically expressed during cell cycle. It is maximally induced as cells enter S phase.
  • Cellular localizationCytoplasmic and Nuclear
  • Alternative names
    • CDK inhibitor p19INK4d antibody
    • CDKN2D antibody
    • cell cycle inhibitor, Nur77 associating protein antibody
    • Cyclin dependent kinase inhibitor 2D antibody
    • Cyclin-dependent kinase 4 inhibitor D antibody
    • Cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4) antibody
    • inhibitor of cyclin-dependent kinase 4d antibody
    • INK4D antibody
    • MGC109660 antibody
    • MGC137547 antibody
    • MGC73010 antibody
    • p19 antibody
    • p19-INK4D antibody
    • p19INK4d antibody
    • Similar to cyclin-dependent kinase inhibitor 2D antibody
    see all

References for Anti-p19 INK4d antibody (ab52735)

ab52735 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab52735.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"