Overview

  • Product nameAnti-p19 INK4d antibody
    See all p19 INK4d primary antibodies
  • Description
    Mouse monoclonal to p19 INK4d
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human) with a 26kDa tag

  • Positive control
    • p19 INK4D transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab77223 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).
ELISA Use at an assay dependent dilution.

Target

  • RelevanceCyclins are important in regulating the cell cycle through their formation of enzymatic complexes with various cyclin dependent kinases. The activity of the cyclin D dependent kinases is controlled by inhibitors such as the INK4 family, which includes INK4a, INK4b, INK4c, and INK4d (p19 INK4d). p19 INK4d is a specific inhibitor of cdk 4 / cdk 6 and is periodically expressed during cell cycle. It is maximally induced as cells enter S phase.
  • Cellular localizationCytoplasmic and Nuclear
  • Database links
  • Alternative names
    • CDK inhibitor p19INK4d antibody
    • CDKN2D antibody
    • cell cycle inhibitor, Nur77 associating protein antibody
    • Cyclin dependent kinase inhibitor 2D antibody
    • Cyclin-dependent kinase 4 inhibitor D antibody
    • Cyclin-dependent kinase inhibitor 2D (p19, inhibits CDK4) antibody
    • inhibitor of cyclin-dependent kinase 4d antibody
    • INK4D antibody
    • MGC109660 antibody
    • MGC137547 antibody
    • MGC73010 antibody
    • p19 antibody
    • p19-INK4D antibody
    • p19INK4d antibody
    • Similar to cyclin-dependent kinase inhibitor 2D antibody
    see all

Anti-p19 INK4d antibody images

  • Anti-p19 INK4d antibody (ab77223) at 5 µg/ml + immunogen at 0.2 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 17 kDa
    Observed band size : 46 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-p19 INK4d antibody (ab77223) at 5 µg/ml

    Lane 1 : p19 INK4d-transfected 293T cell lysate
    Lane 2 : non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
    Developed using the ECL technique

    Predicted band size : 17 kDa
    Observed band size : 17 kDa

References for Anti-p19 INK4d antibody (ab77223)

ab77223 has not yet been referenced specifically in any publications.

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