p19 INK4d overexpression 293T lysate (whole cell) (ab94314)

Overview

  • Product namep19 INK4d overexpression 293T lysate (whole cell)
    See all p19 INK4d lysates
  • Description
    p19 INK4d overexpression lysate
  • General notesab94314 is a 293T cell transfected lysate in which Human p19 INK4d has been transiently over-expressed using a pCMV-p19 INK4d plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundCyclins are important in regulating the cell cycle through their formation of enzymatic complexes with various cyclin dependent kinases. The activity of the cyclin D dependent kinases is controlled by inhibitors such as the INK4 family, which includes INK4a, INK4b, INK4c, and INK4d (p19 INK4d). p19 INK4d is a specific inhibitor of cdk 4 / cdk 6 and is periodically expressed during cell cycle. It is maximally induced as cells enter S phase.

Associated products

Applications

Our Abpromise guarantee covers the use of ab94314 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

p19 INK4d overexpression 293T lysate (whole cell) images

  • ab94314 at 15µg/lane on an SDS-PAGE gel
  • All lanes : Anti-p19 INK4d antibody (ab77223) at 1/500 dilution

    Lane 1 : p19 INK4d overexpression 293T lysate (whole cell) (ab94314)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for p19 INK4d overexpression 293T lysate (whole cell) (ab94314)

ab94314 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"