General notesExtracts have been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA).
To ensure stability, we recommend aliquoting the extracts into single-use fractions and then storing them at -80ºC.
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
BackgroundThe P19 cell line is derived from an embryonal carcinoma induced in a C3H/He strain mouse. The pluripotent P19 cells can be induced to differentiate into neuronal and glial cells in the presence of retinoic acid. Aggregates of P19 cells differentiate into cardiac and skeletal muscle in the presence of dimethyl sulfoxide (DMSO). In the presence of both retinoic acid and DMSO cells develop as if exposed to retinoic acid only. Cells are therfore used in differentiation studies and to study mechanisms of birth defects due to inappropriate apoptosis and retinoid signalling.
References for p19 nuclear extract lysate (ab14880)
has not yet been referenced specifically in any publications.
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