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Overview

  • Product nameAnti-p21 antibody [65]See all p21 primary antibodies ...
  • Description
    Mouse monoclonal [65] to p21
  • Tested applicationsWB, IP more details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant Mouse p21

  • Epitopeab99853 recognizes an epitope within amino acids 90-109 of Mouse p21
  • Positive controlRat or mouse embryo fibroblast cell lines treated with Actinomycin D

Properties

Applications

Our Abpromise guarantee covers the use of ab99853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 3 µg/ml. Predicted molecular weight: 18 kDa. (Chemiluminescence).
IP IP: Use at an assay dependent dilution. Use 2 µg/sample.

Target

  • FunctionMay be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex.
  • Tissue specificityExpressed in all adult human tissues, with 5-fold lower levels observed in the brain.
  • Sequence similaritiesBelongs to the CDI family.
  • DomainThe PIP-box K+4 motif mediates both the interaction with PCNA and the recuitment of the DCX(DTL) complex: while the PIP-box interacts with PCNA, the presence of the K+4 submotif, recruits the DCX(DTL) complex, leading to its ubiquitination.
    The C-terminal is required for nuclear localization of the cyclin D-CDK4 complex.
  • Post-translational
    modifications    Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA. Phosphorylation at Ser-114 by GSK3-beta enhances ubiquitination by the DCX(DTL) complex.
    Ubiquitinated by MKRN1; leading to polyubiquitination and 26S proteasome-dependent degradation. Ubiquitinated by the DCX(DTL) complex, also named CRL4(CDT2) complex, leading to its degradation during S phase or following UV irradiation. Ubiquitination by the DCX(DTL) complex is essential to control replication licensing and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation.
  • Cellular localizationCytoplasm. Nucleus.

    • Target information above from: UniProt accession P38936 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        CAP20 antibodyCDK-interacting protein 1 antibodyCDKI antibody
        CDKN1 antibodyCDKN1A antibodyCDN1A_HUMAN antibodyCIP1 antibodyCyclin Dependent Kinase Inhibitor 1A antibodyCyclin-dependent kinase inhibitor 1 antibodyCyclin-dependent kinase inhibitor 1A (P21) antibodyCyclin-dependent kinase inhibitor 1A (p21, Cip1) antibodyDNA Synthesis Inhibitor antibodyMDA-6 antibodyMDA6 antibodyMelanoma differentiation-associated protein 6 antibodyMelanoma differentiation-associated protein antibodyp21 antibodyP21 protein antibodyp21CIP1 antibodyp21Cip1/Waf1 antibodyp21WAF antibodySDI1 antibodyWAF1 antibodyWild type p53 activated fragment 1 antibodyWildtype p53-activated fragment 1 antibody
      see all

    References for Anti-p21 antibody [65] (ab99853)

    ab99853 has not yet been referenced specifically in any publications.

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