Anti-p21 antibody [AC8.3] (ab118)

Overview

• Product nameAnti-p21 antibody [AC8.3]See all p21 primary antibodies ...
• Description
  Mouse monoclonal [AC8.3] to p21
• Tested applicationsFlow Cyt, Sandwich ELISA, WB more details
• Species reactivity
  Reacts with: Rabbit, Human
  
• Immunogen

  His-tagged human p21 expressed in baculovirus

• General notes

  
  

  Useful as a detection system for rapidly dividing cells.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPhosphate buffered saline
• Concentration information loading...
• PurityProtein A purified
• Primary antibody notes Useful as a detection system for rapidly dividing cells.
• Clonality Monoclonal
• Clone numberAC8.3
• MyelomaSp2
• IsotypeIgG1
• Light chain typeunknown
• Research Areas
  • Cancer
  • Oncoproteins/suppressors
  • Tumor suppressors
  • p53 pathway

  • Cancer
  • Cell cycle
  • Cell cycle inhibitors
  • Cip/kip

  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Cell Cycle Inhibitors
  • Cip/Kip

  • Cell Biology
  • Cell Cycle
  • Cell Cycle Inhibitors
  • Cip / Kip

Applications

Target

• FunctionMay be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex.
• Tissue specificityExpressed in all adult human tissues, with 5-fold lower levels observed in the brain.
• Sequence similaritiesBelongs to the CDI family.
• DomainThe PIP-box K+4 motif mediates both the interaction with PCNA and the recuitment of the DCX(DTL) complex: while the PIP-box interacts with PCNA, the presence of the K+4 submotif, recruits the DCX(DTL) complex, leading to its ubiquitination.
  The C-terminal is required for nuclear localization of the cyclin D-CDK4 complex.
• Post-translational
  modificationsPhosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA. Phosphorylation at Ser-114 by GSK3-beta enhances ubiquitination by the DCX(DTL) complex.
  Ubiquitinated by MKRN1; leading to polyubiquitination and 26S proteasome-dependent degradation. Ubiquitinated by the DCX(DTL) complex, also named CRL4(CDT2) complex, leading to its degradation during S phase or following UV irradiation. Ubiquitination by the DCX(DTL) complex is essential to control replication licensing and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation.
• Cellular localizationCytoplasm. Nucleus.

Target information above from: UniProt accession P38936 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 1026 Human
  • Omim: 116899 Human
  • SwissProt: P38936 Human
  • Unigene: 370771 Human

• Alternative names
  Activating Fragment 1 antibodyActivating Fragment 1 antibodyCAP20 antibody

  Cation chloride cotransporter-interacting protein 1 antibodyCDK Interacting Protein 1 antibodyCDK-interacting protein 1 antibodyCDKI antibodyCDKN 1 antibodyCDKN1 antibodyCDKN1A antibodyCDN1A_HUMAN antibodyCIP1 antibodyCyclin Dependent Kinase Inhibitor 1A antibodyCyclin-dependent kinase inhibitor 1 antibodyCyclin-dependent kinase inhibitor 1A (P21) antibodyCyclin-dependent kinase inhibitor 1A (p21, Cip1) antibodyDNA Synthesis Inhibitor antibodyMDA 6 antibodyMDA-6 antibodyMDA6 antibodyMDA6 antibodyMelanoma Differentiation Associated Protein 6 antibodyMelanoma differentiation-associated protein 6 antibodyMelanoma differentiation-associated protein antibodyp21 antibodyP21 protein antibodyp21CIP1 antibodyp21Cip1/Waf1 antibodyp21WAF antibodyPIC1 antibodySDI1 antibodySLC12A9 antibodyWAF1 antibodyWild type p53 activated fragment 1 antibodyWildtype p53 Activating Fragment 1 antibodyWildtype p53-activated fragment 1 antibody

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Anti-p21 antibody [AC8.3] images

• 

  Sandwich ELISA - p21 antibody [AC8.3] (ab118)
  Standard curve for p21 (Analyte: ab56278); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [AC8.3] to p21 (ab118) at 5µg/ml and Detector Antibody Rabbit polyclonal to p21 (ab18209) at 0.5µg/ml.
• 

  Flow Cytometry-Anti-p21 antibody [AC8.3](ab118)
  Overlay histogram showing HeLa cells stained with ab118 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab118, 2µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.
  
  

Protocols

Protocol Booklet