Anti-p21 antibody (ab102635)

Overview

• Product nameAnti-p21 antibodySee all p21 primary antibodies ...
• Description
  Rabbit polyclonal to p21
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide selected from the S130 region of Human p21, conjugated to KLH.

• Positive controlHeLa cell lysate

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: PBS
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesPurified through a protein A column, followed by peptide affinity purification.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Oncoproteins/suppressors
  • Tumor suppressors
  • p53 pathway

  • Cancer
  • Cell cycle
  • Cell cycle inhibitors
  • Cip/kip

  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Cell Cycle Inhibitors
  • Cip/Kip

  • Cell Biology
  • Cell Cycle
  • Cell Cycle Inhibitors
  • Cip / Kip

Applications

Target

• FunctionMay be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex.
• Tissue specificityExpressed in all adult human tissues, with 5-fold lower levels observed in the brain.
• Sequence similaritiesBelongs to the CDI family.
• DomainThe PIP-box K+4 motif mediates both the interaction with PCNA and the recuitment of the DCX(DTL) complex: while the PIP-box interacts with PCNA, the presence of the K+4 submotif, recruits the DCX(DTL) complex, leading to its ubiquitination.
  The C-terminal is required for nuclear localization of the cyclin D-CDK4 complex.
• Post-translational
  modificationsPhosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA. Phosphorylation at Ser-114 by GSK3-beta enhances ubiquitination by the DCX(DTL) complex.
  Ubiquitinated by MKRN1; leading to polyubiquitination and 26S proteasome-dependent degradation. Ubiquitinated by the DCX(DTL) complex, also named CRL4(CDT2) complex, leading to its degradation during S phase or following UV irradiation. Ubiquitination by the DCX(DTL) complex is essential to control replication licensing and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation.
• Cellular localizationCytoplasm. Nucleus.

Target information above from: UniProt accession P38936 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 1026 Human
  • Omim: 116899 Human
  • SwissProt: P38936 Human
  • Unigene: 370771 Human

• Alternative names
  CAP20 antibodyCDK-interacting protein 1 antibodyCDKI antibody

  CDKN1 antibodyCDKN1A antibodyCDN1A_HUMAN antibodyCIP1 antibodyCyclin Dependent Kinase Inhibitor 1A antibodyCyclin-dependent kinase inhibitor 1 antibodyCyclin-dependent kinase inhibitor 1A (P21) antibodyCyclin-dependent kinase inhibitor 1A (p21, Cip1) antibodyDNA Synthesis Inhibitor antibodyMDA-6 antibodyMDA6 antibodyMelanoma differentiation-associated protein 6 antibodyMelanoma differentiation-associated protein antibodyp21 antibodyP21 protein antibodyp21CIP1 antibodyp21Cip1/Waf1 antibodyp21WAF antibodySDI1 antibodyWAF1 antibodyWild type p53 activated fragment 1 antibodyWildtype p53-activated fragment 1 antibody

  see all

Anti-p21 antibody images

• 

  Western blot - p21 antibody (ab102635)
  Anti-p21 antibody (ab102635) at 1/100 dilution + HeLa cell lysate at 35 µg
  
  Predicted band size : 18 kDa