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Product name
Anti-p21 antibody
See all p21 products (27) ...
Description
Rabbit polyclonal to p21
Specificity
Reacts with p21(also designated WAF1/Cip1)
Tested applications
IHC-Fr, IP, WB, ICC/IF, ICC, Flow Cytmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide: SMTDFYHSKRRLIFSKRKP, corresponding to C terminal amino acids 156-164 of Human p21.
SMTDFYHSKRRLIFSKRKP
Positive control
C32 nuclear lysate. MEF treated 2 µM doxorubicin for 4 hours. Cells treated with adriamycin for 8 hours.
Properties
Form
Liquid
Storage instructions
Store at +4°C. Do not freeze.
Storage buffer
PBS with 0.1% sodium azide
Concentration
Concentration information loading...
Purity
IgG fraction
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> p53 pathway
Cancer >> Cell cycle >> Cell cycle inhibitors >> Cip/kip
Epigenetics and Nuclear Signaling >> Cell cycle >> Cell Cycle Inhibitors >> Cip/Kip
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Cip / Kip
Applications
Show applications key
Our Abpromise guarantee covers the use of ab7960 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: 1/100 - 1/5000. Predicted molecular weight: 18 kDa.
ICC/IF: Use at an assay dependent dilution. See Joers A et al.
ICC: Use at an assay dependent dilution.
Flow Cyt: Use at an assay dependent dilution. See Joers A et al.
Target
Function
May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex.
Tissue specificity
Expressed in all adult human tissues, with 5-fold lower levels observed in the brain.
Sequence similarities
Belongs to the CDI family.
Domain
The PIP-box K+4 motif mediates both the interaction with PCNA and the recuitment of the DCX(DTL) complex: while the PIP-box interacts with PCNA, the presence of the K+4 submotif, recruits the DCX(DTL) complex, leading to its ubiquitination.
The C-terminal is required for nuclear localization of the cyclin D-CDK4 complex.
Post-translational
modifications
Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA. Phosphorylation at Ser-114 by GSK3-beta enhances ubiquitination by the DCX(DTL) complex.
Ubiquitinated by MKRN1; leading to polyubiquitination and 26S proteasome-dependent degradation. Ubiquitinated by the DCX(DTL) complex, also named CRL4(CDT2) complex, leading to its degradation during S phase or following UV irradiation. Ubiquitination by the DCX(DTL) complex is essential to control replication licensing and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation.
Cellular localization
Cytoplasm. Nucleus.
Target information above from: UniProt accessionP38936
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Activating Fragment 1 antibody
- CAP20 antibody
- Cation chloride cotransporter-interacting protein 1 antibody
see all
Database links
- Entrez Gene: 1026 Human
- Entrez Gene: 12575 Mouse
- Entrez Gene: 114851 Rat
- Omim: 116899 Human
- SwissProt: P38936 Human
- SwissProt: P39689 Mouse
- Unigene: 370771 Human
- Unigene: 195663 Mouse
see all
Anti-p21 antibody images:
Western blot - p21 antibody (ab7960)
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 18 kDa
Whole cell lysates (uniduced) were prepared from A431 (1), HL60 (2), and HeLa (3) cells and run on a 8-16% Tris-Glycine gel. ab7960 was applied at a 1:200 dilution for a 1 hour exposure. The secondary antibody was a goat anti-rabbit-HRP 1:5000 (non-Abcam).
Detects cross-reacting bands at ~45KDa and ~75KDa. Signal is more pronounced and appears more specific in induced cells.
Western blot - p21 antibody (ab7960)
All lanes : Anti-p21 antibody (ab7960) at 1/1000 dilution
Lane 1 : Non-adriamycin treated MEF cells - whole cell lysate
Lane 2 : 1 hour adriamycin treated MEF cells -whole cell lysate
Lane 3 : 8 hour adriamycin treated MEF cells -whole cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
HRP conjugated donkey anti-rabbit polyclonal antibody
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 18 kDa
Observed band size : 19 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minute
This image is courtesy of an anonymous Abreview
Western blot - p21 antibody (ab7960)
All lanes : Anti-p21 antibody (ab7960) at 1/500 dilution
Lane 1 : Control siRNA
Lane 2 : p63 siRNA
Lane 3 : p53 siRNA
Lane 4 : p63+p53 siRNA
Lane 5 : p63+p21 siRNA
Secondary
HRP conjugated donkey anti-rabbit IgG at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 18 kDa
Observed band size : 18 kDa
Exposure time : 2 minutes
In this experiment siRNA knockdown of human keratinocytes was carried out for 2 days. A whole cell lysate was prepared using urea lysis buffer and 10ug was loaded per lane. The image shows western blotting with ab after speicific knockdowns and indicates that p21 is upregulated with p63 knockdown but the upregulation is inhibited by double knockdown with p53 or p21 siRNA.
This image is courtesy of an anonymous Abreview
References for Anti-p21 antibody (ab7960)
This product has been referenced in:
- Singh KK et al. BRCA2 Protein Deficiency Exaggerates Doxorubicin-induced Cardiomyocyte Apoptosis and Cardiac Failure. J Biol Chem 287:6604-14 (2012). WB; Mouse.Read more (PubMed: 22157755) »
- Thorne JL et al. Epigenetic control of a VDR-governed feed-forward loop that regulates p21(waf1/cip1) expression and function in non-malignant prostate cells. Nucleic Acids Res 39:2045-56 (2011). WB; Human.Read more (PubMed: 21088000) »
See all 19 publications for this product
Publishing research using ab7960? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"