Overview

  • Product nameAnti-p21 antibody [AC8.3]See all p21 primary antibodies ...
  • Description
    Mouse monoclonal [AC8.3] to p21
  • Tested applicationsFlow Cyt, Sandwich ELISA, WB more details
  • Species reactivity
    Reacts with: Rabbit, Human
  • Immunogen

    His-tagged human p21 expressed in baculovirus

  • General notes


    Useful as a detection system for rapidly dividing cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab118 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Flow Cyt Use 2µg for 106 cells.
Sandwich ELISA Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to p21 (ab18209). For sandwich ELISA, use this antibody as Capture at 5µg/ml with ab18209 as Detection.
WB Use at an assay dependent dilution. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).

Target

  • FunctionMay be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex.
  • Tissue specificityExpressed in all adult human tissues, with 5-fold lower levels observed in the brain.
  • Sequence similaritiesBelongs to the CDI family.
  • DomainThe PIP-box K+4 motif mediates both the interaction with PCNA and the recuitment of the DCX(DTL) complex: while the PIP-box interacts with PCNA, the presence of the K+4 submotif, recruits the DCX(DTL) complex, leading to its ubiquitination.
    The C-terminal is required for nuclear localization of the cyclin D-CDK4 complex.
  • Post-translational
    modifications
    Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA. Phosphorylation at Ser-114 by GSK3-beta enhances ubiquitination by the DCX(DTL) complex.
    Ubiquitinated by MKRN1; leading to polyubiquitination and 26S proteasome-dependent degradation. Ubiquitinated by the DCX(DTL) complex, also named CRL4(CDT2) complex, leading to its degradation during S phase or following UV irradiation. Ubiquitination by the DCX(DTL) complex is essential to control replication licensing and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation.
  • Cellular localizationCytoplasm. Nucleus.
  • Target information above from: UniProt accession P38936 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • Activating Fragment 1 antibody
    • Activating Fragment 1 antibody
    • CAP20 antibody
    • Cation chloride cotransporter-interacting protein 1 antibody
    • CDK Interacting Protein 1 antibody
    • CDK-interacting protein 1 antibody
    • CDKI antibody
    • CDKN 1 antibody
    • CDKN1 antibody
    • CDKN1A antibody
    • CDN1A_HUMAN antibody
    • CIP1 antibody
    • Cyclin Dependent Kinase Inhibitor 1A antibody
    • Cyclin-dependent kinase inhibitor 1 antibody
    • Cyclin-dependent kinase inhibitor 1A (P21) antibody
    • Cyclin-dependent kinase inhibitor 1A (p21, Cip1) antibody
    • DNA Synthesis Inhibitor antibody
    • MDA 6 antibody
    • MDA-6 antibody
    • MDA6 antibody
    • MDA6 antibody
    • Melanoma Differentiation Associated Protein 6 antibody
    • Melanoma differentiation-associated protein 6 antibody
    • Melanoma differentiation-associated protein antibody
    • p21 antibody
    • P21 protein antibody
    • p21CIP1 antibody
    • p21Cip1/Waf1 antibody
    • p21WAF antibody
    • PIC1 antibody
    • SDI1 antibody
    • SLC12A9 antibody
    • WAF1 antibody
    • Wild type p53 activated fragment 1 antibody
    • Wildtype p53 Activating Fragment 1 antibody
    • Wildtype p53-activated fragment 1 antibody
    see all

Anti-p21 antibody [AC8.3] images

  • Standard curve for p21 (Analyte: ab56278); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [AC8.3] to p21 (ab118) at 5µg/ml and Detector Antibody Rabbit polyclonal to p21 (ab18209) at 0.5µg/ml.
  • Overlay histogram showing HeLa cells stained with ab118 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab118, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

Protocols

References for Anti-p21 antibody [AC8.3] (ab118)

This product has been referenced in:
  • Petrides A & Trexler EB Differential output of the high-sensitivity rod photoreceptor: AII amacrine pathway. J Comp Neurol 507:1653-62 (2008). IHC-FrFl ; Rabbit . Read more (PubMed: 18241050) »

See 1 Publication for this product

Product Wall

Unfortunately we still do not have any cross reactivity data. p21 is highly conserved so it has a good chance that it will react, at least across mammals.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"