Anti-p21 antibody [CP74] (Biotin) (ab79467)

Overview

  • Product nameAnti-p21 antibody [CP74] (Biotin)
    See all p21 primary antibodies
  • Description
    Mouse monoclonal [CP74] to p21 (Biotin)
  • ConjugationBiotin
  • Tested applicationsSuitable for: WB, IP, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Full length human recombinant p21 protein

  • EpitopeAmino acids 1-80.
  • Positive control
    • Raji, PC12 cells; human colon cancer tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab79467 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
IHC-P
Flow Cyt
  • Application notesFlow Cyt: Use at an assay dependent dilution.
    IHC-P: 1/50 for 30 min. Staining of formalin-fixed tissue requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for for 10-20 min followed by cooling at RT for 20 min.
    IP: Use at a concentration of 2 µg/ml (Native).
    WB: Use at a concentration of 1 - 2 µg/ml for 2hrs at RT. Predicted molecular weight: 18 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMay be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex.
    • Tissue specificityExpressed in all adult human tissues, with 5-fold lower levels observed in the brain.
    • Sequence similaritiesBelongs to the CDI family.
    • DomainThe PIP-box K+4 motif mediates both the interaction with PCNA and the recuitment of the DCX(DTL) complex: while the PIP-box interacts with PCNA, the presence of the K+4 submotif, recruits the DCX(DTL) complex, leading to its ubiquitination.
      The C-terminal is required for nuclear localization of the cyclin D-CDK4 complex.
    • Post-translational
      modifications
      Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA. Phosphorylation at Ser-114 by GSK3-beta enhances ubiquitination by the DCX(DTL) complex.
      Ubiquitinated by MKRN1; leading to polyubiquitination and 26S proteasome-dependent degradation. Ubiquitinated by the DCX(DTL) complex, also named CRL4(CDT2) complex, leading to its degradation during S phase or following UV irradiation. Ubiquitination by the DCX(DTL) complex is essential to control replication licensing and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation.
    • Cellular localizationCytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • CAP20 antibody
      • CDK-interacting protein 1 antibody
      • CDKI antibody
      • CDKN1 antibody
      • Cdkn1a antibody
      • CDN1A_HUMAN antibody
      • CIP1 antibody
      • Cyclin Dependent Kinase Inhibitor 1A antibody
      • Cyclin-dependent kinase inhibitor 1 antibody
      • Cyclin-dependent kinase inhibitor 1A (P21) antibody
      • Cyclin-dependent kinase inhibitor 1A (p21, Cip1) antibody
      • DNA Synthesis Inhibitor antibody
      • MDA-6 antibody
      • MDA6 antibody
      • Melanoma differentiation-associated protein 6 antibody
      • Melanoma differentiation-associated protein antibody
      • p21 antibody
      • P21 protein antibody
      • p21CIP1 antibody
      • p21Cip1/Waf1 antibody
      • p21WAF antibody
      • PIC1 antibody
      • SDI1 antibody
      • SLC12A9 antibody
      • WAF1 antibody
      • Wild type p53 activated fragment 1 (WAF1) antibody
      • Wild type p53 activated fragment 1 antibody
      • Wildtype p53-activated fragment 1 antibody
      see all

    References for Anti-p21 antibody [CP74] (Biotin) (ab79467)

    ab79467 has not yet been referenced specifically in any publications.

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