Overview

  • Product nameAnti-p21-ARC antibody
    See all p21-ARC primary antibodies
  • Description
    Goat polyclonal to p21-ARC
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide:

    C-RQFMNKSLSGPGQ

    , corresponding to C terminal amino acids 165-177 of Human p21-ARC

  • Positive control
    • WB: Hela, Human Brain and Mouse Brain lysates.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris saline. pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab37916 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa).

Target

  • Relevancep21-ARC is part of a complex implicated in the control of actin polymerization in cells. It belongs to a complex composed of ARP2, ARP3, ARPC1, ARPC2, ARPC3, ARPC4 and ARPC5, collectively known as the ARPC3 family. p21-ARC is implicated as an important regulator of growth cone translocation, especially in the CNS (Strasser et al, 2004).
  • Cellular localizationCytoplasmic; cytoskeleton
  • Database links
  • Alternative names
    • Actin related protein 2/3 complex subunit 3 antibody
    • ARC21 antibody
    • ARP2/3 complex 21 kDa subunit antibody
    • ARP2/3 protein complex subunit p21 antibody
    • ARPC3 antibody
    see all

Anti-p21-ARC antibody images

  • Anti-p21-ARC antibody (ab37916) at 1 µg/ml + Mouse Brain lysate (RIPA buffer 35µg total protein per lane).

    Predicted band size : 20 kDa

References for Anti-p21-ARC antibody (ab37916)

This product has been referenced in:
  • Lippi G  et al. Targeting of the Arpc3 actin nucleation factor by miR-29a/b regulates dendritic spine morphology. J Cell Biol 194:889-904 (2011). WB ; Mouse . Read more (PubMed: 21930776) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample Zebrafish Cell lysate - whole cell (whole larval cell lysate 48-72 hours post fertiliz)
Specification whole larval cell lysate 48-72 hours post fertiliz
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Evgenia Salta

Verified customer

Submitted Nov 12 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"