Overview

  • Product name
  • Description
    Rabbit polyclonal to p21-ARC
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Zebrafish
  • Immunogen

    Recombinant protein fragment with a sequence corresponding to a region within amino acids 1 and 165 of Human p21-ARC (NP_005710).

  • Positive control
    • 293T, A431, H1299, HeLaS3, HepG2, Molt-4 and cell lines. Raji whole cell lysate for WB and endo mitral OVCA tissue for IHC.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7.0
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab96137 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 21 kDa.
IHC-P Use at an assay dependent concentration.

Target

  • Relevance
    p21-ARC is part of a complex implicated in the control of actin polymerization in cells. It belongs to a complex composed of ARP2, ARP3, ARPC1, ARPC2, ARPC3, ARPC4 and ARPC5, collectively known as the ARPC3 family. p21-ARC is implicated as an important regulator of growth cone translocation, especially in the CNS (Strasser et al, 2004).
  • Cellular localization
    Cytoplasmic; cytoskeleton
  • Database links
  • Alternative names
    • Actin related protein 2/3 complex subunit 3 antibody
    • ARC21 antibody
    • ARP2/3 complex 21 kDa subunit antibody
    • ARP2/3 protein complex subunit p21 antibody
    • ARPC3 antibody
    see all

Images

  • Anti-p21-ARC antibody (ab96137) at 1/500 dilution + Raji whole cell lysate at 30 µg

    Predicted band size : 21 kDa
  • ab96137, at 1/100, staining paraffin-embedded endo mitral OVCA by Immunohistochemistry.

References

ab96137 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Breast cancer cell line)
Loading amount
50 µg
Specification
Breast cancer cell line
Gel Running Conditions
Reduced Denaturing (12% and 15%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Otto Cerqueira

Verified customer

Submitted Oct 22 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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