• Nature
  • Amino Acid Sequence


    Our Abpromise guarantee covers the use of ab23370 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Form
    • Additional notes

      - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
      - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
      - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
      - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
      - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

      Information available upon request.

    General Info

    • Alternative names
      • CAP20
      • CDK-interacting protein 1
      • CDKI
      • CDKN1
      • Cdkn1a
      • CDN1A_HUMAN
      • CIP1
      • Cyclin Dependent Kinase Inhibitor 1A
      • Cyclin-dependent kinase inhibitor 1
      • Cyclin-dependent kinase inhibitor 1A (P21)
      • Cyclin-dependent kinase inhibitor 1A (p21, Cip1)
      • DNA Synthesis Inhibitor
      • MDA-6
      • MDA6
      • Melanoma differentiation-associated protein
      • Melanoma differentiation-associated protein 6
      • p21
      • P21 protein
      • p21CIP1
      • p21Cip1/Waf1
      • p21WAF
      • PIC1
      • SDI1
      • SLC12A9
      • WAF1
      • Wild type p53 activated fragment 1
      • Wild type p53 activated fragment 1 (WAF1)
      • Wildtype p53-activated fragment 1
      see all
    • Function
      May be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex.
    • Tissue specificity
      Expressed in all adult human tissues, with 5-fold lower levels observed in the brain.
    • Sequence similarities
      Belongs to the CDI family.
    • Domain
      The PIP-box K+4 motif mediates both the interaction with PCNA and the recuitment of the DCX(DTL) complex: while the PIP-box interacts with PCNA, the presence of the K+4 submotif, recruits the DCX(DTL) complex, leading to its ubiquitination.
      The C-terminal is required for nuclear localization of the cyclin D-CDK4 complex.
    • Post-translational
      Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA. Phosphorylation at Ser-114 by GSK3-beta enhances ubiquitination by the DCX(DTL) complex.
      Ubiquitinated by MKRN1; leading to polyubiquitination and 26S proteasome-dependent degradation. Ubiquitinated by the DCX(DTL) complex, also named CRL4(CDT2) complex, leading to its degradation during S phase or following UV irradiation. Ubiquitination by the DCX(DTL) complex is essential to control replication licensing and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation.
    • Cellular localization
      Cytoplasm. Nucleus.
    • Information by UniProt


    ab23370 has not yet been referenced specifically in any publications.

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