Overview

Description

  • NatureSynthetic
  • Amino Acid Sequence

    Specifications

    Our Abpromise guarantee covers the use of ab23370 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • FormLiquid
    • Additional notes

      - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
      - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
      - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
      - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
      - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

      Information available upon request.

    General Info

    • Alternative names
      • CAP20
      • CDK-interacting protein 1
      • CDKI
      • CDKN1
      • Cdkn1a
      • CDN1A_HUMAN
      • CIP1
      • Cyclin Dependent Kinase Inhibitor 1A
      • Cyclin-dependent kinase inhibitor 1
      • Cyclin-dependent kinase inhibitor 1A (P21)
      • Cyclin-dependent kinase inhibitor 1A (p21, Cip1)
      • DNA Synthesis Inhibitor
      • MDA-6
      • MDA6
      • Melanoma differentiation-associated protein
      • Melanoma differentiation-associated protein 6
      • p21
      • P21 protein
      • p21CIP1
      • p21Cip1/Waf1
      • p21WAF
      • PIC1
      • SDI1
      • SLC12A9
      • WAF1
      • Wild type p53 activated fragment 1
      • Wild type p53 activated fragment 1 (WAF1)
      • Wildtype p53-activated fragment 1
      see all
    • FunctionMay be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex.
    • Tissue specificityExpressed in all adult human tissues, with 5-fold lower levels observed in the brain.
    • Sequence similaritiesBelongs to the CDI family.
    • DomainThe PIP-box K+4 motif mediates both the interaction with PCNA and the recuitment of the DCX(DTL) complex: while the PIP-box interacts with PCNA, the presence of the K+4 submotif, recruits the DCX(DTL) complex, leading to its ubiquitination.
      The C-terminal is required for nuclear localization of the cyclin D-CDK4 complex.
    • Post-translational
      modifications
      Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA. Phosphorylation at Ser-114 by GSK3-beta enhances ubiquitination by the DCX(DTL) complex.
      Ubiquitinated by MKRN1; leading to polyubiquitination and 26S proteasome-dependent degradation. Ubiquitinated by the DCX(DTL) complex, also named CRL4(CDT2) complex, leading to its degradation during S phase or following UV irradiation. Ubiquitination by the DCX(DTL) complex is essential to control replication licensing and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation.
    • Cellular localizationCytoplasm. Nucleus.
    • Information by UniProt

    References for p21 peptide (ab23370)

    ab23370 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab23370.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"