Anti-p21 (phospho T145) antibody (ab39409)

Overview

  • Product nameAnti-p21 (phospho T145) antibodySee all p21 primary antibodies ...
  • Description
    Rabbit polyclonal to p21 (phospho T145)
  • Specificityab39409 detects endogenous levels of p21 only when phosphorylated at Threonine 145.
  • Tested applicationsWB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic phosphopeptide derived from amino acids around the phosphorylation site of Threonine 145 (Human)

  • Positive control
    • HeLa cells treated with EGF.

Properties

Applications

Our Abpromise guarantee covers the use of ab39409 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: 1/10000.
    WB: 1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 18 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionMay be the important intermediate by which p53/TP53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression. Functions in the nuclear localization and assembly of cyclin D-CDK4 complex and promotes its kinase activity towards RB1. At higher stoichiometric ratios, inhibits the kinase activity of the cyclin D-CDK4 complex.
    • Tissue specificityExpressed in all adult human tissues, with 5-fold lower levels observed in the brain.
    • Sequence similaritiesBelongs to the CDI family.
    • DomainThe PIP-box K+4 motif mediates both the interaction with PCNA and the recuitment of the DCX(DTL) complex: while the PIP-box interacts with PCNA, the presence of the K+4 submotif, recruits the DCX(DTL) complex, leading to its ubiquitination.
      The C-terminal is required for nuclear localization of the cyclin D-CDK4 complex.
    • Post-translational
      modifications
      Phosphorylation of Thr-145 by Akt or of Ser-146 by PKC impairs binding to PCNA. Phosphorylation at Ser-114 by GSK3-beta enhances ubiquitination by the DCX(DTL) complex.
      Ubiquitinated by MKRN1; leading to polyubiquitination and 26S proteasome-dependent degradation. Ubiquitinated by the DCX(DTL) complex, also named CRL4(CDT2) complex, leading to its degradation during S phase or following UV irradiation. Ubiquitination by the DCX(DTL) complex is essential to control replication licensing and is PCNA-dependent: interacts with PCNA via its PIP-box, while the presence of the containing the 'K+4' motif in the PIP box, recruit the DCX(DTL) complex, leading to its degradation.
    • Cellular localizationCytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Activating Fragment 1 antibody
      • Activating Fragment 1 antibody
      • CAP20 antibody
      • CAP20 antibody
      • Cation chloride cotransporter-interacting protein 1 antibody
      • CDK Interacting Protein 1 antibody
      • CDK Interacting Protein 1 antibody
      • CDK-interacting protein 1 antibody
      • CDKI antibody
      • CDKN 1 antibody
      • CDKN1 antibody
      • CDKN1 antibody
      • CDKN1A antibody
      • CDKN1A antibody
      • CDN1A_HUMAN antibody
      • CIP1 antibody
      • CIP1 antibody
      • Cyclin Dependent Kinase Inhibitor 1A antibody
      • Cyclin Dependent Kinase Inhibitor 1A antibody
      • Cyclin-dependent kinase inhibitor 1 antibody
      • Cyclin-dependent kinase inhibitor 1A (P21) antibody
      • Cyclin-dependent kinase inhibitor 1A (p21, Cip1) antibody
      • DNA Synthesis Inhibitor antibody
      • MDA 6 antibody
      • MDA-6 antibody
      • MDA6 antibody
      • MDA6 antibody
      • Melanoma Differentiation Associated Protein 6 antibody
      • Melanoma differentiation-associated protein 6 antibody
      • Melanoma differentiation-associated protein antibody
      • p21 antibody
      • p21 antibody
      • P21 protein antibody
      • p21CIP1 antibody
      • p21Cip1/Waf1 antibody
      • p21WAF antibody
      • PIC1 antibody
      • PIC1 antibody
      • SDI1 antibody
      • SDI1 antibody
      • SLC12A9 antibody
      • WAF1 antibody
      • WAF1 antibody
      • Wild type p53 activated fragment 1 antibody
      • Wildtype p53 Activating Fragment 1 antibody
      • Wildtype p53 Activating Fragment 1 antibody
      • Wildtype p53-activated fragment 1 antibody
      see all

    Anti-p21 (phospho T145) antibody images

    • All lanes : Anti-p21 (phospho T145) antibody (ab39409) at 1/1000 dilution

      Lane 1 : Untreated extracts from HeLa cells
      Lane 2 : Extracts from HeLa cells treated with EGF


      Predicted band size : 18 kDa
      Observed band size : 32 kDa (why is the actual band size different from the predicted?)

    References for Anti-p21 (phospho T145) antibody (ab39409)

    ab39409 has not yet been referenced specifically in any publications.

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    Thank you for contacting Abcam. I have looked at the two antibodies you are interested in, ab39409 and ab47300 and since neither has been tested in mouse, we cannot gurantee that they will work for your particular requirments. However, what I can offer...

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