Overview

  • Product name
    Anti-p23 antibody [EPR3846]
    See all p23 primary antibodies
  • Description
    Rabbit monoclonal [EPR3846] to p23
  • Tested applications
    Suitable for: WB, IP, ICC/IFmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues in Human p23.

  • Positive control
    • WB: HeLa, Human spleen, Jurkat and HepG2 cell lysates ICC/IF: HeLa cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab92503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
ICC/IF
  • Application notes
    ICC/IF: 1/100 - 1/250.
    IP: 1/50.
    WB: 1/10000 - 1/50000. Predicted molecular weight: 19 kDa.

    Is unsuitable for Flow Cyt or IHC-P.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Molecular chaperone that localizes to genomic response elements in a hormone-dependent manner and disrupts receptor-mediated transcriptional activation, by promoting disassembly of transcriptional regulatory complexes.
    • Pathway
      Lipid metabolism; prostaglandin biosynthesis.
    • Sequence similarities
      Belongs to the p23/wos2 family.
      Contains 1 CS domain.
    • Cellular localization
      Cytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Co chaperone p23 antibody
      • cPGES antibody
      • Cytosolic prostaglandin E synthase antibody
      • Cytosolic prostaglandin E2 synthase antibody
      • Hsp90 co chaperone antibody
      • Hsp90 co-chaperone antibody
      • P23 antibody
      • Progesterone receptor complex antibody
      • Progesterone receptor complex p23 antibody
      • Prostaglandin E synthase 3 (cytosolic) antibody
      • Prostaglandin E synthase 3 antibody
      • PTGES 3 antibody
      • PTGES3 antibody
      • Sid 3177 antibody
      • TEBP antibody
      • TEBP_HUMAN antibody
      • Telomerase binding protein p23 antibody
      • Telomerase-binding protein p23 antibody
      • Unactive progesterone receptor 23 kD antibody
      see all

    Images

    • All lanes : Anti-p23 antibody [EPR3846] (ab92503) at 1/10000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Human spleen lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP-labeled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 19 kDa
      Observed band size : 23 kDa (why is the actual band size different from the predicted?)
    • Immunofluorescent staining of HeLa cells, using ab92503 at a dilution of 1/100.

    References

    This product has been referenced in:
    • He Y  et al. Ailanthone targets p23 to overcome MDV3100 resistance in castration-resistant prostate cancer. Nat Commun 7:13122 (2016). Read more (PubMed: 27959342) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Hek293)
    Loading amount
    40 µg
    Specification
    Hek293
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted May 12 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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