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Synthetic peptide corresponding to Human p27 KIP 1 aa 153-165 (internal sequence).
Our Abpromise guarantee covers the use of ab45872 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 22 kDa).
Approx 26kDa band observed in lysates of Human Prostate and of Hepatoblastoma cell line HepG2. The observed molecular weight corresponds to earlier findings in literature with different antibodies (PMID: 16169901).
|ELISA||Use at an assay dependent concentration.
Peptide ELISA: Antibody detection limit dilution 1/32,000.
ab45872 at 5µg/ml staining of paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
ab45872 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"