Synthetic peptide corresponding to Human p27 KIP 1 conjugated to Keyhole Limpet Haemocyanin (KLH). Synthetic phosphopeptide derived from human p27 KIP 1 around the phosphorylation site of threonine 187 (E-Q-TP-P-K).
Database link: P46527
Our Abpromise guarantee covers the use of ab75908 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 22 kDa.|
|IHC-P||1/50 - 1/100.|
|ICC/IF||Use a concentration of 5 µg/ml.|
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab75908 (left) at 1/50 dilution or the same antibody preincubated with blocking peptide (right).
ICC/IF image of ab75908 stained A431 cells (ab7909). The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75908, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.