Anti-p27 KIP 1 antibody [DCS-72.F6] (ab3928)

Overview

  • Product nameAnti-p27 KIP 1 antibody [DCS-72.F6]
    See all p27 KIP 1 primary antibodies
  • Description
    Mouse monoclonal [DCS-72.F6] to p27 KIP 1
  • SpecificityThis antibody is specific to a protein of 27 kDa known as p27 / Kip1 a cell cycle regulatory mitotic inhibitor. This antibody does not show any cross reactivity with other related mitotic inhibitors.
  • Tested applicationsIHC-P, IHC-Fr, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Recombinant full length protein (Mouse).

  • EpitopeBetween amino acids 83-204 of p27.
  • Positive control
    • Colon carcinoma.

Properties

Applications

Our Abpromise guarantee covers the use of ab3928 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
IP 1/50.
WB 1/50 - 1/100. Predicted molecular weight: 22 kDa.
IF Use at an assay dependent concentration. PubMed: 24658003

Target

  • FunctionImportant regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Forms a complex with cyclin type D-CDK4 complexes and is involved in the assembly, stability, and modulation of CCND1-CDK4 complex activation. Acts either as an inhibitor or an activator of cyclin type D-CDK4 complexes depending on its phosphorylation state and/or stoichometry.
  • Tissue specificityExpressed in all tissues tested. Highest levels in skeletal muscle, lowest in liver and kidney.
  • Involvement in diseaseDefects in CDKN1B are the cause of multiple endocrine neoplasia type 4 (MEN4) [MIM:610755]. Multiple endocrine neoplasia (MEN) syndromes are inherited cancer syndromes of the thyroid. MEN4 is a MEN-like syndrome with a phenotypic overlap of both MEN1 and MEN2.
  • Sequence similaritiesBelongs to the CDI family.
  • DomainA peptide sequence containing only AA 28-79 retains substantial Kip1 cyclin A/CDK2 inhibitory activity.
  • Post-translational
    modifications
    Phosphorylated; phosphorylation occurs on serine, threonine and tyrosine residues. Phosphorylation on Ser-10 is the major site of phosphorylation in resting cells, takes place at the G(0)-G(1) phase and leads to protein stability. Phosphorylation on other sites is greatly enhanced by mitogens, growth factors, cMYC and in certain cancer cell lines. The phosphorylated form found in the cytoplasm is inactivate. Phosphorylation on Thr-198 is required for interaction with 14-3-3 proteins. Phosphorylation on Thr-187, by CDK2 leads to protein ubiquitination and proteasomal degradation. Tyrosine phosphorylation promotes this process. Phosphorylation by PKB/AKT1 can be suppressed by LY294002, an inhibitor of the catalytic subunit of PI3K. Phosphorylation on Tyr-88 and Tyr-89 has no effect on binding CDK2, but is required for binding CDK4. Dephosphorylated on tyrosine residues by G-CSF.
    Ubiquitinated; in the cytoplasm by the KPC complex (composed of RNF123/KPC1 and UBAC1/KPC2) and, in the nucleus, by SCF(SKP2). The latter requires prior phosphorylation on Thr-187. Ubiquitinated; by a TRIM21-containing SCF(SKP2)-like complex; leads to its degradation.
    Subject to degradation in the lysosome. Interaction with SNX6 promotes lysosomal degradation.
  • Cellular localizationNucleus. Cytoplasm. Endosome. Nuclear and cytoplasmic in quiescent cells. AKT-or RSK-mediated phosphorylation on Thr-198, binds 14-3-3, translocates to the cytoplasm and promotes cell cycle progression. Mitogen-activated UHMK1 phosphorylation on Ser-10 also results in translocation to the cytoplasm and cell cycle progression. Phosphorylation on Ser-10 facilitates nuclear export. Translocates to the nucleus on phosphorylation of Tyr-88 and Tyr-89. Colocalizes at the endosome with SNX6 and this leads to lysosomal degradation.
  • Information by UniProt
  • Database links
  • Alternative names
    • AA408329 antibody
    • AI843786 antibody
    • Cdki1b antibody
    • CDKN 1B antibody
    • CDKN 4 antibody
    • CDKN1B antibody
    • CDKN4 antibody
    • CDN1B_HUMAN antibody
    • Cyclin Dependent Kinase Inhibitor 1B antibody
    • Cyclin dependent kinase inhibitor p27 antibody
    • Cyclin-dependent kinase inhibitor 1B (p27, Kip1) antibody
    • Cyclin-dependent kinase inhibitor 1B antibody
    • Cyclin-dependent kinase inhibitor p27 antibody
    • Cyclin-dependent kinase inhibitor p27 Kip1 antibody
    • KIP 1 antibody
    • KIP1 antibody
    • MEN1B antibody
    • MEN4 antibody
    • OTTHUMP00000195098 antibody
    • OTTHUMP00000195099 antibody
    • p27 antibody
    • p27 Kip1 antibody
    • P27-like cyclin-dependent kinase inhibitor antibody
    • P27KIP1 antibody
    see all

Anti-p27 KIP 1 antibody [DCS-72.F6] images

  • ab3928 at a 1/200 dilution staining rat brain (dentate gyrus, sub-ventricular zone) tissue sections by Immunohistochemistry (Frozen sections). The antibody was added to the tissue sections for 24 hours and then detected using an Alexa Fluor ® 488 conjugated rabbit anti-mouse antibody.

    This image is courtesy of an Abreview.

    See Abreview

  • ab3928 staining human colon carcinoma by IHC-P.

References for Anti-p27 KIP 1 antibody [DCS-72.F6] (ab3928)

This product has been referenced in:
  • Gómez-Herreros F  et al. TDP2 protects transcription from abortive topoisomerase activity and is required for normal neural function. Nat Genet 46:516-21 (2014). IF ; Human . Read more (PubMed: 24658003) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Liver)
Specification Liver
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated
Permeabilization No
Blocking step H2O2 as blocking agent for 5 minute(s) · Concentration: 0.03% · Temperature: RT°C
Username

Dr. Saira Khalid

Verified customer

Submitted Feb 01 2010

I'm sorry to hear you are having a problem with ab3928. If I understand your problem correctly you are seeing staining in the cytoplasm only but not in the nucleus. As you are probably aware p27 KIP 1 can be in the nucleus or cytoplasm and can also...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Brain (dentate gyrus))
Specification Brain (dentate gyrus)
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted May 16 2006

The concentration is 100 ug/ml. Please let us know if you have any additional questions.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"