• Product nameAnti-P2RX7 antibody
    See all P2RX7 primary antibodies
  • Description
    Rabbit polyclonal to P2RX7
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 550 to the C-terminus of Rat P2RX7.

  • Positive control
    • This antibody gave a positive signal in Rat Hippocampus tissue lysate in western blot, and FFPE rat brain tissue sections in IHCP.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab109054 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 68 kDa (predicted molecular weight: 68 kDa).
IHC-P Use a concentration of 0.1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • FunctionReceptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells.
  • Tissue specificityWidely expressed with highest levels in brain and immune tissues.
  • Sequence similaritiesBelongs to the P2X receptor family.
  • Post-translational
    Phosphorylation results in its inactivation.
    ADP-ribosylation at Arg-125 is necessary and sufficient to activate P2RX7 and gate the channel.
    Palmitoylation of several cysteines in the C-terminal cytoplasmic tail is required for efficient localization to cell surface.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP receptor antibody
    • P2rx7 antibody
    • P2RX7_HUMAN antibody
    • P2X purinoceptor 7 antibody
    • P2X7 antibody
    • P2Z receptor antibody
    • Purinergic receptor antibody
    • purinergic receptor P2X, ligand gated ion channel, 7 antibody
    see all

Anti-P2RX7 antibody images

  • Anti-P2RX7 antibody (ab109054) at 1 µg/ml + Rat Hippocampus Tissue Lysate at 10 µg

    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 68 kDa
    Observed band size : 68 kDa
    Additional bands at : 20 kDa,78 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 90 seconds
  • IHC image of ab109054 staining in rat brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab109054, 0.1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-P2RX7 antibody (ab109054)

ab109054 has not yet been referenced specifically in any publications.

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