Overview

  • Product name
  • Description
    Goat polyclonal to P2RX7
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Guinea pig, Dog
  • Immunogen

    Synthetic peptide:

    YETNKVTRIQSMNY

    by a Cysteine residue linker, corresponding to N terminal amino acids 13-26 of Human P2RX7 (NP_002553.2)

  • Positive control
    • Human brain frontal cortex lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab93354 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 37, 70 kDa (predicted molecular weight: 69 kDa).
IHC-P Use a concentration of 3 - 6 µg/ml.

Target

  • Function
    Receptor for ATP that acts as a ligand-gated ion channel. Responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules. Could function in both fast synaptic transmission and the ATP-mediated lysis of antigen-presenting cells.
  • Tissue specificity
    Widely expressed with highest levels in brain and immune tissues.
  • Sequence similarities
    Belongs to the P2X receptor family.
  • Post-translational
    modifications
    Phosphorylation results in its inactivation.
    ADP-ribosylation at Arg-125 is necessary and sufficient to activate P2RX7 and gate the channel.
    Palmitoylation of several cysteines in the C-terminal cytoplasmic tail is required for efficient localization to cell surface.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ATP receptor antibody
    • P2rx7 antibody
    • P2RX7_HUMAN antibody
    • P2X purinoceptor 7 antibody
    • P2X7 antibody
    • P2Z receptor antibody
    • Purinergic receptor antibody
    • purinergic receptor P2X, ligand gated ion channel, 7 antibody
    see all

Images

  • All lanes : Anti-P2RX7 antibody (ab93354) at 0.3 µg/ml

    Lane 1 : Human brain frontal cortex lysate
    Lane 2 : Human brain frontal cortex lysate with Immunising peptide

    Lysates/proteins at 35 µg per lane.

    Developed using the ECL technique

    Predicted band size : 69 kDa
    Observed band size : 37,70 kDa (why is the actual band size different from the predicted?)
  • ab87597 at 3.8 µg/ml staining P2RX7 in Human brain cortex. Steamed antigen retrieval with citrate buffer pH 6 was performed. Antibody was revealed with AP-staining.

References

ab93354 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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