Overview

  • Product nameAnti-P2X2 antibody
    See all P2X2 primary antibodies
  • Description
    Rabbit polyclonal to P2X2
  • Tested applicationsSuitable for: WB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    A region within synthetic peptide: YPKFHFSKGN IADRTDGYLK RCTFHEASDL YCPIFKLGFI VEKAGESFTE, corresponding to internal sequence amino acids 181-230 of Human P2X2. Peptide available as ab95915.

  • Positive control
    • Jurkat cell lysate, Human kidney.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab48864 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.6 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

Target

  • FunctionIon channel gated by extracellular ATP involved in a variety of cellular responses, such as excitatory postsynaptic responses in sensory neurons, neuromuscular junctions (NMJ) formation, hearing, perception of taste and peristalsis. In the inner ear, regulates sound transduction and auditory neurotransmission, outer hair cell electromotility, inner ear gap junctions, and K(+) recycling. Mediates synaptic transmission between neurons and from neurons to smooth muscle.
  • Involvement in diseaseDeafness, autosomal dominant, 41
  • Sequence similaritiesBelongs to the P2X receptor family.
  • Cellular localizationCell membrane. Localizes to the apical membranes of hair cells in the organ of Corti.
  • Information by UniProt
  • Database links
  • FormThere are 7 isoforms produced by alternative splicing.
  • Alternative names
    • ATP receptor antibody
    • MGC129601 antibody
    • P2RX 2 antibody
    • P2RX2 antibody
    • P2RX2_HUMAN antibody
    • P2X purinoceptor 2 antibody
    • P2X Receptor subunit 2 antibody
    • P2X2 antibody
    • P2X2a antibody
    • Purinergic receptor antibody
    • Purinergic receptor P2X ligand gated ion channel 2 antibody
    • Purinergic receptor P2X2 isoform A antibody
    • Purinergic receptor P2X2 isoform B antibody
    • Purinergic receptor P2X2 isoform C antibody
    • Purinergic receptor P2X2 isoform D antibody
    • Purinergic receptor P2X2 isoform H antibody
    • Purinergic receptor P2X2 isoform I antibody
    see all

Anti-P2X2 antibody images

  • Anti-P2X2 antibody (ab48864) at 0.6 µg/ml (in 5% skimmed milk/PBS) + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 40 kDa
    Observed band size : 40 kDa
    Gel concentration 12%.
  • ab48864 at 4.0 µg/ml staining Human kidney by immunohistochemistry, paraffin embedded section. Magnification x400. Arrows indicate staining of renal tubule epithelial cells.

References for Anti-P2X2 antibody (ab48864)

This product has been referenced in:
  • Anastasiades PG  et al. GABAergic interneurons form transient layer-specific circuits in early postnatal neocortex. Nat Commun 7:10584 (2016). IHC-FoFr . Read more (PubMed: 26843463) »
  • Graham CE  et al. A corticotropin-releasing factor system expressed in the cochlea modulates hearing sensitivity and protects against noise-induced hearing loss. Neurobiol Dis 38:246-58 (2010). WB ; Mouse . Read more (PubMed: 20109547) »

See all 2 Publications for this product

Product Wall

Thank you for your inquiry. To our knowledge, ab10266 has not yet been tested in flow cytometry. It is possible that this product may be suitable for your experiment since it has been successfully used in ICC, but we don't have any data regarding its ...

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