The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 20 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at an assay dependent concentration. Predicted molecular weight: 42 kDa. PubMed: 24396418
FunctionReceptor for lysophosphatidic acid (LPA), a mediator of diverse cellular activities. Transduces a signal by increasing the intracellular calcium ions and by stimulating adenylyl cyclase activity. The rank order of potency for agonists of this receptor is 1-oleoyl- > 1-stearoyl- > 1-palmitoyl- > 1-myristoyl- > 1-alkyl- > 1-alkenyl-LPA.
Tissue specificityHigh expression in ovary. Not detected in the brain regions thalamus, putamen, caudate, frontal cortex, pons, hypothalamus and hippocampus.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
ab115454, at 20 µg/ml, staining P2Y9 in formalin-fixed, paraffin-embedded Human Brain, Cortex tissue by Immunohistochemistry, using a biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.
References for Anti-P2Y9 antibody (ab115454)
This product has been referenced in:
Feng Y & Liu JF Expression of lysophosphatidic acid receptors in the human lower esophageal sphincter. Exp Ther Med7:423-428 (2014).
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