You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

p38 MAPK alpha (pT180/pY182) + total p38 MAPK alpha ELISA Kit (ab126453)

Submit an Abreview Submit a Question

Overview

  • Product namep38 MAPK alpha (pT180/pY182) + total p38 MAPK alpha ELISA KitSee all p38 MAPKalpha (phospho Thr180/Tyr182)+ total p38 MAPKalpha kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell Lysate
  • Assay typeSandwich
  • Assay time
    5h 00m
  • Species reactivity
    Reacts with: Mouse, Human
  • Product overview

    ab126453 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cell lysates. By determining phosphorylated p38 alpha protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blot analysis.

    This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-p38 alpha (Thr180/Tyr182) and total p38 alpha (help normalize the results of phospho-p38 alpha from different cell lysate being compared). An anti-p38 alpha (Thr180/Tyr182) (half plate, red marker on left side) and anti-total p38 alpha antibody (half plate, black marker on right side) has been coated onto a 96-well plate. Samples are pipetted into the wells and phosphorylated (left side) and total (right side) p38 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti p38 alpha is used to detect phosphorylated or total p38 alpha. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of p38 alpha (Thr180/Tyr182) or total p38 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsSandwich ELISA more details

Properties

  • FunctionResponds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.
  • Tissue specificityBrain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications    Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm. Nucleus.

    • Target information above from: UniProt accession Q16539 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        CSAID binding proteinCSAID-binding proteinCsaids binding protein
        CSBPCSBP1CSBP2CSPB1Cytokine suppressive anti-inflammatory drug-binding proteinEXIPMAP kinase 14MAP kinase MXI2MAP kinase p38 alphaMAPK 14MAPK14MAX-interacting protein 2Mitogen-activated protein kinase 14Mitogen-activated protein kinase p38 alphaMK14_HUMANMxi2p38p38 MAP kinasep38 mitogen activated protein kinasep38alphap38alpha ExipPRKM14PRKM15RKSAPK2AStress activated protein kinase 2A
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab126453 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      Sandwich ELISA sELISA

      p38 MAPK alpha (pT180/pY182) + total p38 MAPK alpha ELISA Kit images

      • Sandwich ELISA - p38 alpha (pT180/pY182) + total p38 alpha ELISA Kit (ab126453)
        Sandwich ELISA - p38 alpha (pT180/pY182) + total p38 alpha ELISA Kit (ab126453)
        HeLa cells were treated with Anisomycin at 37°C for 10 min. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA.
      • Sandwich ELISA - p38 alpha (pT180/pY182) + total p38 alpha ELISA Kit (ab126453)
        Sandwich ELISA - p38 alpha (pT180/pY182) + total p38 alpha ELISA Kit (ab126453)
        HeLa cells were treated or untreated with Anisomycin for 10 min at 37°C. Cell lysates were analyzed using this phosphoELISA.
      • Western blot - p38 alpha (pT180/pY182) + total p38 alpha ELISA Kit (ab126453)
        Western blot - p38 alpha (pT180/pY182) + total p38 alpha ELISA Kit (ab126453)
        HeLa cells were treated or untreated with Anisomycin for 10 min at 37°C. Cell lysates were analyzed by Western Blot.

      Protocols

      References for p38 MAPK alpha (pT180/pY182) + total p38 MAPK alpha ELISA Kit (ab126453)

      ab126453 has not yet been referenced specifically in any publications.

      Publishing research using ab126453 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab126453 Submit a Question for ab126453

      There are currently no Abreviews or Questions for ab126453.
      Please use the links above to contact us or submit feedback about this product.

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"