The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 41 kDa).
Responds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.
Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. Contains 1 protein kinase domain.
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme. Phosphorylated upon DNA damage, probably by ATM or ATR.
Paraffin-embedded human colon carcinoma tissue stained for p38 using ab59461 at 1/10000 dilution in immunohistochemical analysis. Secondary Antibody: Biotin Goat Anti-Mouse at 1/2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT.
Western blot - Anti-p38 antibody [9F12] (ab59461)
All lanes : Anti-p38 antibody [9F12] (ab59461) at 1/1000 dilution
Lane 1 : A431 (human epidermoid carcinoma cell line) cell lysate Lane 2 : A549 (human lung carcinoma cell line) cell lysate Lane 3 : HCT 116 (human colorectal carcinoma cell line) cell lysate Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate Lane 5 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate Lane 6 : HepG2 (human liver hepatocellular carcinoma cell line) cell lysate Lane 7 : Hl-60 (human promyelocytic leukemia cell line) cell lysate Lane 8 : HUVEC (human umbilical vein endothelial cell line) cell lysate Lane 9 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate Lane 10 : MCF7 (human breast adenocarcinoma cell line) cell lysate Lane 11 : PC-3 (human prostate adenocarcinoma cell line) cell lysate Lane 12 : T98G cell lysate Lane 13 : Rat brain lysate