![Western blot - p38 antibody [Y122] (ab32142) image](#ab32142_1.JPG)
![Immunohistochemistry (Paraffin-embedded sections) - p38 antibody [Y122] (ab32142) image](#ab32142-2.JPG)
![Immunofluorescence - p38 antibody [Y122] (ab32142) image](#ab32142-3.JPG)
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Product name
Anti-p38 antibody [Y122]
See all p38 products (20) ...
Description
Rabbit monoclonal [Y122] to p38
Specificity
This antibody recognises p38. It is predicted to react with splice isoform CSBP1 according to sequence homology. Mouse cross reactivity has been tested by WB and IHC, Rat cross reactivity by WB only.
Tested applications
ICC/IF, WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
A synthetic peptide corresponding to residues near the C terminus of human p38.
Positive control
WB: Hela, Jurkat, K562 and MCF7 cell lyastes. IHC-P: Human skin carcinoma. ICC/IF: MCF7 cells.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Concentration
Concentration information loading...
Clonality
Monoclonal
Clone number
Y122
Isotype
IgG
Research areas
Immunology >> Innate Immunity >> TLR Signaling
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> MAPK Pathway
Applications
Show applications key
Our Abpromise guarantee covers the use of ab32142 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF
ICC/IF: 1/100 - 1/250.ICC/IF: 1/100 - 1/250.
WB: 1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 41 kDa).
IHC-P: 1/100 - 1/250.
Application notes
Is unsuitable for ,Flow Cyt or IP.
Target
Function
Responds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.
Tissue specificity
Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.
Domain
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Post-translational
modifications
Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Cytoplasm. Nucleus.
Target information above from: UniProt accessionQ16539
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CSAID Binding Protein 1 antibody
- CSAID binding protein antibody
- CSAID-binding protein antibody
see all
Database links
- Entrez Gene: 1432 Human
- Entrez Gene: 26416 Mouse
- Entrez Gene: 81649 Rat
- GenBank: NM_001315 Human
- GenBank: NM_139012 Human
- GenBank: NM_011951 Mouse
- GenBank: NM_031020 Rat
- Omim: 600289 Human
see all
Anti-p38 antibody [Y122] images:
Western blot - p38 antibody [Y122] (ab32142)
![Western blot - p38 antibody [Y122] (ab32142)](/ps/datasheet/Images/32/ab32142/ab32142_1.JPG)
All lanes : Anti-p38 antibody [Y122] (ab32142) at 1/50000 dilution
Lane 1 : Hela cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : K562 cell lysate
Lane 4 : MCF7 cell lysate
Predicted band size : 41 kDa
Observed band size : 42 kDa (why is the actual band size different from the predicted?)
10 ug protein per lane
Immunohistochemistry (Paraffin-embedded sections) - p38 antibody [Y122] (ab32142)
![Immunohistochemistry (Paraffin-embedded sections) - p38 antibody [Y122] (ab32142)](/ps/datasheet/Images/32/ab32142/ab32142-2.JPG)
Ab32142, at a 1/100 dilution, staining p38 in paraffin embedded human skin carcinoma tissue by immunohistochemistry.
Immunofluorescence - p38 antibody [Y122] (ab32142)
![Immunofluorescence - p38 antibody [Y122] (ab32142)](/ps/datasheet/Images/32/ab32142/ab32142-3.JPG)
Ab32142, at a 1/100 dilution, staining p38 in MCF7 cells by Immunofluorescence.
References for Anti-p38 antibody [Y122] (ab32142)
ab32142 has not yet been referenced specifically in any publications.
Publishing research using ab32142? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"