• Product nameAnti-p38 antibody
    See all p38 primary antibodies
  • Description
    Rabbit polyclonal to p38
  • Specificityab27986 detects total p38 MAP kinase. Does not cross react with either JNK/SAPK or p44/42 MAP kinase.
  • Tested applicationsSuitable for: WB, IP, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Human)(Peptide available as ab53314.)

  • Positive control
    • Jurkat cell lysate


Associated products


Our Abpromise guarantee covers the use of ab27986 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesIHC-Fr: Use at an assay dependent dilution.
    IP: Use at a concentration of 10 - 20 µg/ml.
    WB: Use at a concentration of 1 - 2 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 41 kDa).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionResponds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.
    • Tissue specificityBrain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.
    • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
      Contains 1 protein kinase domain.
    • DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
    • Post-translational
      Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme.
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationCytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • CSAID Binding Protein 1 antibody
      • CSAID binding protein antibody
      • CSAID-binding protein antibody
      • Csaids binding protein antibody
      • CSBP 1 antibody
      • CSBP 2 antibody
      • CSBP antibody
      • CSBP1 antibody
      • CSBP2 antibody
      • CSPB1 antibody
      • Cytokine suppressive anti-inflammatory drug-binding protein antibody
      • EXIP antibody
      • MAP kinase 14 antibody
      • MAP kinase MXI2 antibody
      • MAP kinase p38 alpha antibody
      • MAPK 14 antibody
      • MAPK14 antibody
      • MAX interacting protein 2 antibody
      • MAX-interacting protein 2 antibody
      • Mitogen Activated Protein Kinase 14 antibody
      • Mitogen activated protein kinase p38 alpha antibody
      • Mitogen-activated protein kinase 14 antibody
      • Mitogen-activated protein kinase p38 alpha antibody
      • MK14_HUMAN antibody
      • Mxi 2 antibody
      • MXI2 antibody
      • p38 ALPHA antibody
      • p38 antibody
      • p38 MAP kinase antibody
      • p38 MAPK antibody
      • p38 mitogen activated protein kinase antibody
      • p38ALPHA antibody
      • p38alpha Exip antibody
      • PRKM14 antibody
      • PRKM15 antibody
      • RK antibody
      • SAPK2A antibody
      see all

    Anti-p38 antibody images

    References for Anti-p38 antibody (ab27986)

    This product has been referenced in:
    • Kang K  et al. Carnosic acid slows photoreceptor degeneration in the Pde6b(rd10) mouse model of retinitis pigmentosa. Sci Rep 6:22632 (2016). WB . Read more (PubMed: 26961159) »
    • Lamarca A  et al. Uridine 5'-Triphosphate Promotes In Vitro Schwannoma Cell Migration through Matrix Metalloproteinase-2 Activation. PLoS One 9:e98998 (2014). WB ; Rat . Read more (PubMed: 24905332) »

    See all 3 Publications for this product

    Product Wall

    Application Western blot
    Sample Mouse Tissue lysate - whole (retina)
    Gel Running Conditions Non-reduced Non-Denaturing (Native)
    Loading amount 40 µg
    Specification retina
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C

    Abcam user community

    Verified customer

    Submitted Jul 06 2015

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
    Sample Mouse Cell (retina)
    Specification retina
    Permeabilization Yes - 0.5% Triton X-100
    Fixative Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted Jan 05 2015