Overview

  • Product nameAnti-p38 antibody [M138]
    See all p38 primary antibodies
  • Description
    Mouse monoclonal [M138] to p38
  • Tested applicationsSuitable for: IHC-P, Flow Cyt, WB, ELISA, ICCmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Dog, Human, African Green Monkey, Syrian Hamster
  • Immunogen

    Recombinant fragment corresponding to Rat p38 (C terminal). Identical to human and mouse.

  • Epitopeab31828 recognises an epitope located in the C terminal region of p38.
  • Positive control
    • A431 cells, Jurkat cells, HeLa cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.1% BSA
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesPurified with protein A affinity chromatography.
  • ClonalityMonoclonal
  • Clone numberM138
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab31828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/20 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/100.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB 1/1000. Predicted molecular weight: 41 kDa.
ELISA 1/2000.

Peptide ELISA only

ICC 1/200.

Target

  • FunctionResponds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.
  • Tissue specificityBrain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications
    Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CSAID Binding Protein 1 antibody
    • CSAID binding protein antibody
    • CSAID-binding protein antibody
    • Csaids binding protein antibody
    • CSBP 1 antibody
    • CSBP 2 antibody
    • CSBP antibody
    • CSBP1 antibody
    • CSBP2 antibody
    • CSPB1 antibody
    • Cytokine suppressive anti-inflammatory drug-binding protein antibody
    • EXIP antibody
    • MAP kinase 14 antibody
    • MAP kinase MXI2 antibody
    • MAP kinase p38 alpha antibody
    • MAPK 14 antibody
    • MAPK14 antibody
    • MAX interacting protein 2 antibody
    • MAX-interacting protein 2 antibody
    • Mitogen Activated Protein Kinase 14 antibody
    • Mitogen activated protein kinase p38 alpha antibody
    • Mitogen-activated protein kinase 14 antibody
    • Mitogen-activated protein kinase p38 alpha antibody
    • MK14_HUMAN antibody
    • Mxi 2 antibody
    • MXI2 antibody
    • p38 ALPHA antibody
    • p38 antibody
    • p38 MAP kinase antibody
    • p38 MAPK antibody
    • p38 mitogen activated protein kinase antibody
    • p38ALPHA antibody
    • p38alpha Exip antibody
    • PRKM14 antibody
    • PRKM15 antibody
    • RK antibody
    • SAPK2A antibody
    see all

Anti-p38 antibody [M138] images



  • Predicted band size : 41 kDa

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: p38 knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: Jurkat cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab31828 observed at 40 kDa. Red - loading control, ab181602, observed at 37 kDa.

    ab31828 was shown to specifically react with p38 when p38 knockout samples were used. Wild-type and p38 knockout samples were subjected to SDS-PAGE. ab31828 and ab181602 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) ab216777 secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging.

  • IHC image of ab31828 staining p38 in normal human esophagus formalin-fixed paraffin-embedded tissue sections*, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab31828, 1/50 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre



  • Predicted band size : 41 kDa
    Western blot analysis of A431 cells serum starved overnight (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2 & 4). The blot was probed with anti-p38alpha (lanes 1 & 2) or anti-p38 (T180/Y182) (lanes 3-4). Lanes 5-7 shows a blot of A431 cells treated with pervanadate and probed with anti-p38 (T180/Y182) in the presence of no peptide (lane 5), phospho-ERK1 (T202/Y204) peptide (lane 6) or phoshpo-p38 (T180/Y182) peptide (lane 7).
  • Immunocytochemical labeling of activated p38 MAPK in pervanadate-treated mouse with ab31828. The cells were labeled with mouse monoclonal p38α MAPK and p38 MAPK antibodies, then the antibodies were detected using appropriate secondary antibodies conjugated to Cy3.

  • Overlay histogram showing A431 cells stained with ab31828 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab21828, 1:100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1:500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in A431 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

References for Anti-p38 antibody [M138] (ab31828)

This product has been referenced in:
  • Ruess DA  et al. HDACi Valproic Acid (VPA) and Suberoylanilide Hydroxamic Acid (SAHA) Delay but Fail to Protect against Warm Hepatic Ischemia-Reperfusion Injury. PLoS One 11:e0161233 (2016). WB ; Rat . Read more (PubMed: 27513861) »
  • Damgaard RB  et al. The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity. Cell 166:1215-1230.e20 (2016). WB ; Mouse . Read more (PubMed: 27523608) »

See all 8 Publications for this product

Product Wall

Application Western blot
Sample Rat Tissue lysate - other (muscle)
Gel Running Conditions Reduced Denaturing (9)
Loading amount 40 µg
Specification muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Nov 01 2016

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (NIH-3T3 cells)
Permeabilization No
Specification NIH-3T3 cells
Blocking step 1% Donkey Serum + 1% BSA + 0.1% Triton X-100, in PBS as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative Paraformaldehyde
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Submitted Nov 02 2015

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (10)
Sample Human Cell lysate - other (HeLa)
Specification HeLa
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Aug 25 2014

Application Western blot
Loading amount 100 µg
Gel Running Conditions Reduced Denaturing (10)
Sample Mouse Tissue lysate - whole (Mouse whole embryo (E10.5) extract)
Specification Mouse whole embryo (E10.5) extract
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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Submitted Aug 25 2014

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Application Western blot
Sample Syrian Hamster Cell lysate - whole cell (BHK cells)
Loading amount 100 µg
Specification BHK cells
Gel Running Conditions Reduced Denaturing (8% Tris-Glycine gel)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted May 14 2013

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Application Western blot
Sample African Green Monkey Cell lysate - whole cell (COS cells)
Loading amount 100 µg
Specification COS cells
Gel Running Conditions Reduced Denaturing (8% Tris-Glycine gel)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted May 10 2013

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Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Loading amount 100 µg
Specification HeLa cells
Gel Running Conditions Reduced Denaturing (8% Tris-Glycine gel)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted May 07 2013

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Application Western blot
Sample Cow Cell lysate - whole cell (MDBK cells)
Loading amount 100 µg
Specification MDBK cells
Gel Running Conditions Reduced Denaturing (8% Tris-Glycine gel)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Apr 10 2013

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Application Western blot
Sample Dog Cell lysate - whole cell (MDCK cells)
Loading amount 100 µg
Specification MDCK cells
Gel Running Conditions Reduced Denaturing (8% Tris-Glycine gel)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Apr 10 2013

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Application Western blot
Sample Rat Cell lysate - whole cell (NRK cells)
Loading amount 50 µg
Specification NRK cells
Gel Running Conditions Reduced Denaturing (8% Tris-Glycine gel)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Apr 10 2013

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"