Overview

  • Product nameAnti-p38 antibody [Y122]
    See all p38 primary antibodies
  • Description
    Rabbit monoclonal [Y122] to p38
  • SpecificityThis antibody recognises p38. It is predicted to react with splice isoform CSBP1 according to sequence homology. Mouse cross reactivity has been tested by WB and IHC, Rat cross reactivity by WB only.
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues near the C terminus of human p38.

  • Positive control
    • WB: Hela, Jurkat, K562 and MCF7 cell lyastes. IHC-P: Human skin carcinoma. ICC/IF: MCF7 cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Alternative versions available:

    Anti-p38 antibody (Alexa Fluor® 488) [Y122] (ab193897)

    Anti-p38 antibody (Alexa Fluor® 647) [Y122] (ab196646)

     

Properties

Applications

Our Abpromise guarantee covers the use of ab32142 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/250.
WB 1/1000 - 1/10000. Detects a band of approximately 42 kDa (predicted molecular weight: 41 kDa).
IHC-P Use at an assay dependent concentration.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionResponds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.
    • Tissue specificityBrain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.
    • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
      Contains 1 protein kinase domain.
    • DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
    • Post-translational
      modifications
      Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme.
      Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationCytoplasm. Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • CSAID Binding Protein 1 antibody
      • CSAID binding protein antibody
      • CSAID-binding protein antibody
      • Csaids binding protein antibody
      • CSBP 1 antibody
      • CSBP 2 antibody
      • CSBP antibody
      • CSBP1 antibody
      • CSBP2 antibody
      • CSPB1 antibody
      • Cytokine suppressive anti-inflammatory drug-binding protein antibody
      • EXIP antibody
      • MAP kinase 14 antibody
      • MAP kinase MXI2 antibody
      • MAP kinase p38 alpha antibody
      • MAPK 14 antibody
      • MAPK14 antibody
      • MAX interacting protein 2 antibody
      • MAX-interacting protein 2 antibody
      • Mitogen Activated Protein Kinase 14 antibody
      • Mitogen activated protein kinase p38 alpha antibody
      • Mitogen-activated protein kinase 14 antibody
      • Mitogen-activated protein kinase p38 alpha antibody
      • MK14_HUMAN antibody
      • Mxi 2 antibody
      • MXI2 antibody
      • p38 ALPHA antibody
      • p38 antibody
      • p38 MAP kinase antibody
      • p38 MAPK antibody
      • p38 mitogen activated protein kinase antibody
      • p38ALPHA antibody
      • p38alpha Exip antibody
      • PRKM14 antibody
      • PRKM15 antibody
      • RK antibody
      • SAPK2A antibody
      see all

    Anti-p38 antibody [Y122] images



    • Predicted band size : 41 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: p38 knockout HAP1 cell lysate (20 µg)
      Lane 3: HeLa cell lysate (20 µg)
      Lane 4: Jurkat cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab32142 observed at 40 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab32142 was shown to specifically react with p38 when p38 knockout samples were used. Wild-type and p38 knockout samples were subjected to SDS-PAGE. ab32142 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) cells labelling p38 (green) with purified ab32142 at 1/250. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Nuclei were counterstained with DAPI (blue).

      Secondary Only Control: PBS was used instead of the primary antibody as the negative control.

    • All lanes : Anti-p38 antibody [Y122] (ab32142) at 1/50000 dilution

      Lane 1 : Hela cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : K562 cell lysate
      Lane 4 : MCF7 cell lysate


      Predicted band size : 41 kDa
      Observed band size : 42 kDa (why is the actual band size different from the predicted?)
      10 ug protein per lane
    • Ab32142, at a 1/100 dilution, staining p38 in paraffin embedded human skin carcinoma tissue by immunohistochemistry.

    References for Anti-p38 antibody [Y122] (ab32142)

    This product has been referenced in:
    • Zhang J  et al. Comparisons of ethanol extracts of chinese propolis (poplar type) and poplar gums based on the antioxidant activities and molecular mechanism. Evid Based Complement Alternat Med 2015:307594 (2015). Mouse . Read more (PubMed: 25802536) »
    • Yan T  et al. Luteolin inhibits behavioral sensitization by blocking methamphetamine-induced MAPK pathway activation in the caudate putamen in mice. PLoS One 9:e98981 (2014). WB ; Human . Read more (PubMed: 24901319) »

    See all 7 Publications for this product

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    DISCOUNT CODE: *** Expiration date: April 20th, 2012 I am very pleased to hear you would like to accept our offer and test ab32142 in canine. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submi...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"