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ab126452 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human and mouse cell lysates. By determining phosphorylated p38 alpha protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human and mouse phospho-p38 alpha (Thr180/Tyr182). An anti-phospho-p38 alpha (Thr180/Tyr182) antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and phosphorylated p38 alpha (Thr180/Tyr182) present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-p38 alpha antibody is used to detect phosphorylated p38 alpha. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of p38 alpha (Thr180/Tyr182) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get higher sensitivity in only 90 minutes with p38 MAPK alpha (pT180/Y182) ELISA Kit (ab176649) from our SimpleStep ELISA® range.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|2x Cell lysate buffer||1 x 5ml|
|5X Assay Diluent||1 x 15ml|
|Detection Antibody p38 alpha||2 vials|
|HRP-conjugated anti-rabbit IgG||1 x 25µl|
|p38 alpha Microplate (12 strips x 8 wells) coated with anti-phospho-p38 alpha (T180/Y182) antibody||1 unit|
|Positive Control: lyophilized powder from HeLa cell lysate||2 vials|
|Stop Solution||1 x 8ml|
|TMB Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab126452 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab126452 has not yet been referenced specifically in any publications.