Overview

  • Product name
    p38 MAPK alpha (pT180/pY182) ELISA Kit
    See all p38 alpha (pT180/pY182) kits
  • Detection method
    Colorimetric
  • Sample type
    Cell Lysate
  • Assay type
    Semi-quantitative
  • Assay time
    5h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse, Human
  • Product overview

    ab126452 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in human and mouse cell lysates. By determining phosphorylated p38 alpha protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blotting analysis. 


    This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human and mouse phospho-p38 alpha (Thr180/Tyr182). An anti-phospho-p38 alpha (Thr180/Tyr182) antibody has been coated onto a 96-well plate. Samples are pipetted into the wells and phosphorylated p38 alpha (Thr180/Tyr182) present in a sample is bound to the wells by the immobilized antibody. The wells are washed and anti-p38 alpha antibody is used to detect phosphorylated p38 alpha. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of p38 alpha (Thr180/Tyr182) bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


    Get higher sensitivity in only 90 minutes with p38 MAPK alpha (pT180/Y182) ELISA Kit (ab176649) from our SimpleStep ELISA® range.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab126452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • HeLa cells were treated with Anisomycin at 37°C for 10 min. Solubilize cells at 4 x 107 cells/ml in Cell Lysate Buffer. Serial dilutions of lysates were analyzed in this ELISA.
  • HeLa cells were treated or untreated with Anisomycin for 10 min at 37°C. Cell lysates were analyzed using this phosphoELISA.
  • HeLa cells were treated or untreated with Anisomycin for 10 min at 37°C. Cell lysates were analyzed by Western Blot.

Protocols

References

ab126452 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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