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Save time with a 90-minute ELISA protocol. Try p38 MAPK alpha (pT180/Y182 + Total) ELISA Kit (ab176664) from our SimpleStep ELISA® range.
ab126453 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cell lysates. By determining phosphorylated p38 alpha protein in your experimental model system, you can verify pathway activation in your cell lysates. You can simultaneously measure numerous different cell lysates without spending excess time and effort in performing a Western Blot analysis.
This Sandwich ELISA kit is an in vitro enzyme-linked immunosorbent assay for the measurement of human phospho-p38 alpha (Thr180/Tyr182) and total p38 alpha (help normalize the results of phospho-p38 alpha from different cell lysate being compared). An anti-p38 alpha (Thr180/Tyr182) (half plate, red marker on left side) and anti-total p38 alpha antibody (half plate, black marker on right side) has been coated onto a 96-well plate. Samples are pipetted into the wells and phosphorylated (left side) and total (right side) p38 alpha present in a sample is bound to the wells by the immobilized antibody. The wells are washed and rabbit anti p38 alpha is used to detect phosphorylated or total p38 alpha. After washing away unbound antibody, HRP-conjugated anti-rabbit IgG is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of p38 alpha (Thr180/Tyr182) or total p38 alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|2X Cell Lysate Buffer||1 x 5ml|
|5X Assay Diluent||1 x 15ml|
|Detection Antibody p38 alpha||2 vials|
|HRP-conjugated anti-rabbit IgG: 1000x HRP-conjugated anti-rabbit IgG concentrate||1 x 25µl|
|p38 alpha Microplate (12 strips x 8 wells)||1 unit|
|Positive Control: lyophilized powder from HeLa cell lysate||2 vials|
|Stop Solution||1 x 8ml|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab126453 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
ab126453 has not yet been referenced specifically in any publications.