p38 MAPK alpha (pT180/Y182) PhosphoTracer ELISA Kit (ab119638)
- Product namep38 MAPK alpha (pT180/Y182) PhosphoTracer ELISA KitSee all p38 alpha (pT180/pY182) kits ...
- Detection methodFluorescent
- Tests1 x 96 well plate
- Sample typeCell culture extracts
- Assay typeSemi-quantitative
- Sensitivity0.1 ng/ml
- Range0.1 ng/ml - 250 ng/ml
- Assay time2h 0m
- Assay durationOne step assay
- Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
- Product overview
PhosphoTracer assays use a traditional immuno-sandwich format, but with a major difference both the analyte and the assay reagents are added to the PhosphoTracer assay microplate at the same time. After a short incubation period, unbound assay reagents and analytes are washed away, and immuno-complexes containing both antibodies are detected. The process can take as little as 60 minutes to complete.
PhosphoTracer kits also allow a higher degree of assay flexibility. In contrast to other ELISA formats, no antibodies are present on the assay microplate itself, so assays for several different targets can be performed in different wells on the same microplate. Simply mix the lysate with your target reagents of choice, using the microplate configuration of your choice.
A whole new way of performing cellular assays, PhosphoTracer takes the hard work out of running a standard ELISA, while still giving the high quality results expected from a sandwich immunoassay. Fully self-contained kits are supplied in convenient 96-well packs. Simple to use and highly sensitive PhosphoTracer kits are designed to get results, fast.
Abcam’s PhosphoTracer p38 MAPKalpha assay kits detect endogenous levels of p38 MAPKalpha (GenBank Accession NP_001306) in cellular lysates. Phospho-p38 MAPKalpha (pT180/Y182) assay kits detect p38 MAPKalpha only when phosphorylated at Thr180/Tyr182. Total p38 MAPKalpha assay kits detect p38 MAPKalpha irrespective of phosphorylation status.
The substrate used with the HRP conjugated detection antibody is a combination of 10-Acetyl-3,7-dihydroxyphenoxazine (ADHP) (wavelength exc/em = 530-540nm / 590-600nm), a highly sensitive and stable substrate for HRP) and ADHP Dilution Buffer (a stabilized H2O2 solution). Learn more about the fluorogenic substrate, ADHP.
- Tested applicationsSandwich ELISA more details
- Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 96-well PhosphoTracer assay plate (stripwell) 1 unit Adherent plate seal 2 units ADHP (100X) 1 x 120µl ADHP Dilution Buffer 1 x 15ml Assay Control Lysate (lyophilized) 1 x 0.25ml Enhancer Solution 1 x 1ml Lysis Buffer (5X) 1 x 15ml Mouse monoclonal Phospho-p38 MAPK (Thr180/Tyr182) (96 assay points) 1 x 3ml Rabbit polyclonal p38 MAPKalpha (HRP) (96 assay points) 1 x 3ml Stop Solution 1 x 2ml Wash Buffer (10X) 1 x 15ml
- RelevanceThis protein is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
- Cellular localizationCytoplasmic and Nuclear
- CSAID-binding protein
- Cytokine suppressive anti-inflammatory drug-binding protein
- MAP kinase 14
- MAP kinase MXI2
- MAP kinase p38 alpha
- MAPK 14
- MAX-interacting protein 2
- Mitogen-activated protein kinase 14
- Mitogen-activated protein kinase p38 alpha
- Stress-activated protein kinase 2a
Our Abpromise guarantee covers the use of ab119638 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||sELISA. Tested in HeLa, THP1, Jurkat|
p38 MAPK alpha (pT180/Y182) PhosphoTracer ELISA Kit images
Using Western blot, a significant stimulation of p38 MAPKalpha phosphorylation at Thr180/Tyr182 is detected in HEK293 cells treated with anisomycin for 30 minutes (+) compared with untreated cells (-), while no change in total p38 MAPKalpha levels is observed.
Using the p38 MAPKalpha assay kits, a significant stimulation of p38 MAPKalpha phosphorylation at Thr180/Tyr182 is detected in HEK293 cells treated with anisomycin for 30 minutes (+) compared with untreated cells (-), while no change in total p38 MAPKalpha levels is observed.
Using the PhosphoTracer p38 MAPKalpha (pT180/Y182) assay, the length of assay incubation time that was required to detect recombinant phospho-p38 MAPKalpha was examined. After 15 mins, phospho-p38 MAPKalpha concentrations of approximately 4 ng/mL were readily detected, while after 1 hour incubation, the limit of detection was approximately 1 ng/mL, and with a much improved assay window. Incubation times longer than one hour showed a slight improvement on assay performance.
PhosphoTracer p38 MAPKalpha (pT180/Y182) ELISA Kit (ab119638) used in Sandwich ELISA.
References for p38 MAPK alpha (pT180/Y182) PhosphoTracer ELISA Kit (ab119638)
ab119638 has not yet been referenced specifically in any publications.