p38 MAPK alpha (Thr180/Tyr182) In-Cell ELISA Kit (ab126425)
- Product namep38 MAPK alpha (Thr180/Tyr182) In-Cell ELISA Kit
- Tests1 x 96 well plate
- Sample typeAdherent cells
- Assay typeCell-based
- Assay time5h 10m
- Species reactivityReacts with: Mouse, Rat, Human
- Product overview
ab126425 is a very rapid, convenient and sensitive assay kit that can monitor the activation or function of important biological pathways in cells. It can be used for measuring the relative amount of p38 MAPK (Thr180/Tyr182) phosphorylation and screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators in cultured human, mouse and rat cell lines. By determining p38 MAPK protein phosphorylation in your experimental model system, you can verify pathway activation in your cell lines without spending excess time and effort in preparing cell lysate and performing an analysis of Western Blot.
In the p38 MAPK (Thr180/Tyr182) In-Cell ELISA Kit, cells are seeded into a 96 well tissue culture plate. The cells are fixed after various treatments, inhibitors or activators. After blocking, Anti-Phospho-p38 MAPK (Thr180/Tyr182) or Anti-p38 MAPK (primary antibody) is pipetted into the wells and incubated. The wells are washed, and HRP-conjugated anti-mouse IgG (secondary antibody) is added to the wells. The wells are washed again, a TMB substrate solution is added to the wells and color develops in proportion to the amount of protein. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- Tested applicationsIn-Cell ELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests Anti-Mouse IgG Concentrate (Item I) 1 x 10µl Blocking Solution (5x) (Item F) 1 x 20ml Fixing Solution (Item D) 1 x 30ml Microplate (Item A) 1 unit Mouse Anti-p38 MAPK Concentrate (Item H) 1 x 7µl Mouse Anti-Phospho-p38 MAPK (Thr180/Tyr182) Concentrate (Item G) 1 x 7µl Quenching Buffer Concentrate (Item E) 1 x 2ml Stop Solution (Item K) 1 x 14ml TMB One-Step Substrate Reagent (Item J) 1 x 12ml Wash Buffer Concentrate A (20x) (Item B) 1 x 30ml Wash Buffer Concentrate B (20x) (Item C) 1 x 30ml
- Research Areas
Contains 1 protein kinase domain.
modificationsDually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme.
Phosphorylated upon DNA damage, probably by ATM or ATR.
- CSAID-binding proteinCsaids binding proteinCSBP
- CSBP1CSBP2CSPB1Cytokine suppressive anti-inflammatory drug-binding proteinEXIPMAP kinase 14MAP kinase MXI2MAP kinase p38 alphaMAPK 14MAPK14MAX-interacting protein 2Mitogen-activated protein kinase 14Mitogen-activated protein kinase p38 alphaMK14_HUMANMxi2p38p38 MAP kinasep38 mitogen activated protein kinasep38alphap38alpha ExipPRKM14PRKM15RKSAPK2AStress activated protein kinase 2A
Our Abpromise guarantee covers the use of ab126425 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|In-Cell ELISA||In-Cell ELISA|
p38 MAPK alpha (Thr180/Tyr182) In-Cell ELISA Kit images
HeLa cells were stimulated by different concentrations of anisomycin for 15 minutes at 37°C.
HeLa cells were stimulated by different concentrations of anisomycin for 1 hour at 37°C.
Western blot analysis of extracts from 1 µg/ml Anisomycin treated HeLa cells. Phospho-p38 MAPK (Thr180/Tyr182) and Anti-p38 MAPK antibodies from ab126425 were used in both detection assays.
References for p38 MAPK alpha (Thr180/Tyr182) In-Cell ELISA Kit (ab126425)
ab126425 has not yet been referenced specifically in any publications.