![Western blot - p38 (phospho T180) antibody [E229] (ab32557) image](#ab32557-1.JPG)
![Dot Blot - p38 (phospho T180 + Y182) antibody [E229] (ab32557) image](#ab32557_2.JPG)
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Product name
Anti-p38 (phospho T180 + Y182) antibody [E229]
See all p38 products (20) ...
Description
Rabbit monoclonal [E229] to p38 (phospho T180 + Y182)
Specificity
This antibody only detects p38 MAPK phosphorylated on threonine 180 and tyrosine 182.
Tested applications
ICC, IP, Dot Blot, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
A synthetic phospho peptide corresponding to residues surrounding Thr180 and Tyr182 of human p38.
Positive control
Hela cell lysate.
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
Concentration
Concentration information loading...
Clonality
Monoclonal
Clone number
E229
Isotype
IgG
Research areas
Immunology >> Innate Immunity >> TLR Signaling
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> MAPK Pathway
Applications
Show applications key
Our Abpromise guarantee covers the use of ab32557 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC
IP
- 1 Image
Dot Blot
Application notes
Dot: 1/1000.
ICC: 1/100.
IP: 1/10.
WB: 1/1000. Detects a band of approximately 42 kDa (predicted molecular weight: 41 kDa).
Is unsuitable for FACS or IHC.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Responds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.
Tissue specificity
Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain.
Domain
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
Post-translational
modifications
Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme.
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Cytoplasm. Nucleus.
Target information above from: UniProt accessionQ16539
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CSAID Binding Protein 1 antibody
- CSAID binding protein antibody
- CSAID-binding protein antibody
see all
Database links
- Entrez Gene: 1432 Human
- Entrez Gene: 26416 Mouse
- Entrez Gene: 81649 Rat
- GenBank: NM_001315 Human
- GenBank: NM_139012 Human
- GenBank: NM_011951 Mouse
- GenBank: NM_031020 Rat
- Omim: 600289 Human
see all
Anti-p38 (phospho T180 + Y182) antibody [E229] images:
Western blot - p38 (phospho T180) antibody [E229] (ab32557)
![Western blot - p38 (phospho T180) antibody [E229] (ab32557)](/ps/datasheet/Images/32/ab32557/ab32557-1.JPG)
All lanes : Anti-p38 (phospho T180 + Y182) antibody [E229] (ab32557) at 1/1000 dilution
Lane 1 : Hela cell lysate, untreated
Lane 2 : Hela cell lysate, treated with Anisomycin
Predicted band size : 41 kDa
Observed band size : 42 kDa (why is the actual band size different from the predicted?)
Dot Blot - p38 (phospho T180 + Y182) antibody [E229] (ab32557)
![Dot Blot - p38 (phospho T180 + Y182) antibody [E229] (ab32557)](/ps/datasheet/Images/32/ab32557/ab32557_2.JPG)
Ab32557, at a 1/1000 dilution, staining p38 by Dot Blot.
(A) 50ng of immunogen phosphopeptide.
(B) 50ng of non phospho peptide.
References for Anti-p38 (phospho T180 + Y182) antibody [E229] (ab32557)
This product has been referenced in:
- Wang L et al. Concentration-dependent effects of transforming growth factor ß1 on corneal wound healing. Mol Vis 17:2835-46 (2011). ICC/IF; Human.Read more (PubMed: 22128231) »
- Séïté JF et al. IVIg modulates BCR signaling through CD22 and promotes apoptosis in mature human B lymphocytes. Blood 116:1698-704 (2010). WB; Human.Read more (PubMed: 20516366) »
See all 4 publications for this product
Publishing research using ab32557? Please let us know so that we can cite the reference in this datasheet
![p38 (phospho T180 + Y182) antibody [E229] for WB in Human (32557)](/ps/Reviews/Images/ab9645_48480.jpg)
![p38 (phospho T180 + Y182) antibody [E229] for WB in Mouse (32557)](/ps/Reviews/Images/ab9646_48479.jpg)
![Anti-p38 (phospho T180 + Y182) antibody [E229] for Western blot in Mouse (32557)](/ps/datasheet/images/32/ab32557/p38-Primary-antibodies-ab32557-5.jpg)
![p38 (phospho T180 + Y182) antibody [E229] for WB in Human (32557)](/ps/Reviews/Images/ab12860_73455.jpg)
![Anti-p38 (phospho T180 + Y182) antibody [E229] for Western blot in Human (32557)](/ps/datasheet/images/32/ab32557/p38-Primary-antibodies-ab32557-9.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"