Anti-p38 (phospho T180 + Y182) antibody [M139] (ab45381)
Key features and details
- Mouse monoclonal [M139] to p38 (phospho T180 + Y182)
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG1
Overview
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Product name
Anti-p38 (phospho T180 + Y182) antibody [M139]
See all p38 primary antibodies -
Description
Mouse monoclonal [M139] to p38 (phospho T180 + Y182) -
Host species
Mouse -
Specificity
This antibody detects a 42 kDa protein corresponding to the apparent molecular mass of p38a. This peptide sequence is highly conserved in the p38ß,g, and d MAPKs, and is identical in human and mouse p38a. -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Rat p38 (phospho T180 + Y182) conjugated to Keyhole Limpet Haemocyanin (KLH). Clone M139 was generated from a phospho-p38α (Thr-180/Tyr-182) synthetic peptide (coupled to KLH) corresponding to amino acid residues around threonine 180 and tyrosine 182 of rat p38α.
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Positive control
- Pervanadate treated human Jurkat and A431 cells and anisomycin treated human HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Mouse monoclonal purified with protein A chromatography. -
Clonality
Monoclonal -
Clone number
M139 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab45381 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (3) |
1/1000. Predicted molecular weight: 42 kDa.
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Notes |
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WB
1/1000. Predicted molecular weight: 42 kDa. |
Target
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Function
Responds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis. -
Tissue specificity
Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney. -
Sequence similarities
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
Contains 1 protein kinase domain. -
Domain
The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. -
Post-translational
modificationsDually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme.
Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 1432 Human
- Entrez Gene: 26416 Mouse
- Omim: 600289 Human
- SwissProt: Q16539 Human
- SwissProt: P47811 Mouse
- Unigene: 485233 Human
- Unigene: 311337 Mouse
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Alternative names
- CSAID Binding Protein 1 antibody
- CSAID binding protein antibody
- CSAID-binding protein antibody
see all
Images
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Serum starved HepG2 cells were incubated at 37°C for 30 minutes with vehicle control (0 μM) and different concentrations of (±)-naringenin (ab120958). Increased expression of p38 (phospho T180 + Y182) (
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with
μg /ml and ab8227 at 1/1000 dilution overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP (ab97040) at 1/10000 and visualised using ECL development solution. -
All lanes : Anti-p38 (phospho T180 + Y182) antibody [M139] (ab45381) at 1/1000 dilution
Lane 1 : A431 cells serum starved overnight
Lane 2 : A431 cells treated with pervanadate (1 mM) for 30 minutes
Lane 3 : A431 cells treated with pervanadate (1 mM) for 30 minutes with No peptide
Lane 4 : A431 cells treated with pervanadate (1 mM) for 30 minutes with phospho-ERK1 (T202/Y204) peptide
Lane 5 : A431 cells treated with pervanadate (1 mM) for 30 minutes with phoshpo-p38 (T180/Y182) peptide
Lysates/proteins at 20 µg per lane.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (31)
ab45381 has been referenced in 31 publications.
- Sun B et al. Irisin reduces bone fracture by facilitating osteogenesis and antagonizing TGF-β/Smad signaling in a growing mouse model of osteogenesis imperfecta. J Orthop Translat 38:175-189 (2023). PubMed: 36439629
- Chen Y et al. PP1A prevents ROS-induced pyroptosis by inhibiting MAPK/caspase-3 in mouse adipose tissue. FEBS J 289:3839-3853 (2022). PubMed: 35080339
- Nikooie R et al. Lactate regulates autophagy through ROS-mediated activation of ERK1/2/m-TOR/p-70S6K pathway in skeletal muscle. J Cell Commun Signal 15:107-123 (2021). PubMed: 33398722
- Jing J et al. Oxidized-LDL inhibits testosterone biosynthesis by affecting mitochondrial function and the p38 MAPK/COX-2 signaling pathway in Leydig cells. Cell Death Dis 11:626 (2020). PubMed: 32796811
- Dai F et al. Calponin 3 is associated with poor prognosis and regulates proliferation and metastasis in osteosarcoma. Aging (Albany NY) 12:14037-14049 (2020). PubMed: 32667904