Anti-p38 (phospho Y182 + T180) antibody (ab4822)

Overview

• Product nameAnti-p38 (phospho Y182 + T180) antibodySee all p38 primary antibodies ...
• Description
  Rabbit polyclonal to p38 (phospho Y182 + T180)
• SpecificityReacts with p38 alpha, beta and gamma.
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Mouse, Rat, Dog, Human, Carp, Monkey
  
• Immunogen

  Synthetic peptide (Human) derived from the region of human p38 that contains threonine 180 and tyrosine 182. This region is conserved among many species including human, rat, mouse, dog, and carp.

• Positive controlHEK 293 cells +/- UV irradiation treatment and PC12 cells +/- Sorbitol.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.05% Sodium Azide
  Constituents: 50% Glycerol, PBS, 1mg/ml BSA (IgG, protease free). pH 7.3
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesPurified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using i) non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated p38, and ii) a JNK-derived peptide that is phosphorylated at threonine 183 and tyrosine 185. The final product is generated by affinity chromatography using a p38-derived peptide that is phosphorylated at threonine 180 and tyrosine 182.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Protein Phosphorylation
  • Ser / Thr Kinases
  • MAPK Pathway

  • Immunology
  • Innate Immunity
  • TLR Signaling

Applications

Target

• FunctionResponds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.
• Tissue specificityBrain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.
• Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
  Contains 1 protein kinase domain.
• DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
• Post-translational
  modificationsDually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme.
  Phosphorylated upon DNA damage, probably by ATM or ATR.
• Cellular localizationCytoplasm. Nucleus.

Target information above from: UniProt accession Q16539 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 403856 Dog
  • Entrez Gene: 1432 Human
  • Entrez Gene: 26416 Mouse
  • Entrez Gene: 81649 Rat
  • GenBank: NM_001315 Human
  • GenBank: NM_139012 Human
  • GenBank: NM_011951 Mouse
  • GenBank: NM_031020 Rat

  • Omim: 600289 Human
  • SwissProt: O02812 Dog
  • SwissProt: Q16539 Human
  • SwissProt: Q5U421 Mouse
  • SwissProt: P47811 Mouse
  • SwissProt: Q56A33 Rat
  • SwissProt: P70618 Rat
  • Unigene: 485233 Human
  • Unigene: 311337 Mouse
  • Unigene: 88085 Rat

  see all

• Alternative names
  CSAID Binding Protein 1 antibodyCSAID binding protein antibodyCSAID-binding protein antibody

  Csaids binding protein antibodyCSBP 1 antibodyCSBP 2 antibodyCSBP antibodyCSBP1 antibodyCSBP2 antibodyCSPB 1 antibodyCSPB1 antibodyCytokine suppressive anti inflammatory drug binding protein antibodyCytokine suppressive anti-inflammatory drug-binding protein antibodyEXIP antibodyMAP kinase 14 antibodyMAP kinase MXI2 antibodyMAP kinase p38 alpha antibodyMAPK 14 antibodyMAPK14 antibodyMAX interacting protein 2 antibodyMAX-interacting protein 2 antibodyMitogen Activated Protein Kinase 14 antibodyMitogen activated protein kinase p38 alpha antibodyMitogen-activated protein kinase 14 antibodyMitogen-activated protein kinase p38 alpha antibodyMK14_HUMAN antibodyMxi 2 antibodyMxi2 antibodyp38 ALPHA antibodyp38 antibodyp38 MAP kinase antibodyp38 MAPK antibodyp38 mitogen activated protein kinase antibodyp38ALPHA antibodyp38alpha Exip antibodyPRKM14 antibodyPRKM15 antibodyRK antibodySAPK 2A antibodySAPK2A antibodyStress Activated Protein Kinase 2A antibody

  see all

Anti-p38 (phospho Y182 + T180) antibody images

• 

  Western blot - p38 (phospho Y182 + T180) antibody (ab4822)
  
  
  Predicted band size : 38 kDa
  
  
  Peptide Competition: Extracts prepared from HEK 293 cells treated with UV irradiation were resolved on a 10% Tris-glycine gel and transferred to nitrocellulose. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with 0.50 µg/mL ab4822 for two hours at room temperature in a 3% BSA-TBST buffer, following its prior incubation with: the peptide immunogen (1), a generic phosphothreonine containing peptide (2), a generic phosphotyrosine-containing peptide (3), the non-phosphorylated peptide corresponding to the phosphopeptide (4), no peptide (5), the phosphorylated peptide derived from the corresponding region of JNK 1 & 2 (6), and, the phosphorylated peptide derived from the corresponding region of ERK 1 & 2 (7). After washing, membranes were incubated with goat F(ab')2 antirabbit IgG alkaline phosphatase and the signal was detected using the Tropix WesternStar method. The data show that only the phosphopeptide corresponding to this site blocks the antibody signal, demonstrating the specificity of ab4822 for the dually phosphorylated form of this protein.

Protocols

• Peptide Competition Experiment
• Western blotting protocol