Anti-p38 (phospho T180 + Y182) antibody (ab4822)

Overview

  • Product name
    Anti-p38 (phospho T180 + Y182) antibody
    See all p38 primary antibodies
  • Description
    Rabbit polyclonal to p38 (phospho T180 + Y182)
  • Tested applications
    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse, Dog, Carp, Monkey
  • Immunogen

    Synthetic peptide corresponding to Human p38 (phospho T180 + Y182). p38 is dually phosphorylated and therefore fully activated by MEK3 and MEK6 on threonine 180 and tyrosine 182 within the activation loop.
    Database link: Q16539
    (Peptide available as ab5253)

  • Positive control
    • HEK 293 cells +/- UV irradiation treatment and PC12 cells +/- Sorbitol.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.30
    Preservative: 0.05% Sodium azide
    Constituents: 50% PBS, 50% Glycerol, 0.1% BSA

    BSA is IgG and protease free. PBS without Mg2+ and Ca2+.
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using i) non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated p38, and ii) a JNK-derived peptide that is phosphorylated at threonine 183 and tyrosine 185. The final product is generated by affinity chromatography using a p38-derived peptide that is phosphorylated at threonine 180 and tyrosine 182.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab4822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/100.
ICC/IF 1/250.
WB 1/1000. Predicted molecular weight: 38 kDa.

Target

  • Function
    Responds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.
  • Tissue specificity
    Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Domain
    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications
    Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CSAID Binding Protein 1 antibody
    • CSAID binding protein antibody
    • CSAID-binding protein antibody
    • Csaids binding protein antibody
    • CSBP 1 antibody
    • CSBP 2 antibody
    • CSBP antibody
    • CSBP1 antibody
    • CSBP2 antibody
    • CSPB1 antibody
    • Cytokine suppressive anti-inflammatory drug-binding protein antibody
    • EXIP antibody
    • MAP kinase 14 antibody
    • MAP kinase MXI2 antibody
    • MAP kinase p38 alpha antibody
    • MAPK 14 antibody
    • MAPK14 antibody
    • MAX interacting protein 2 antibody
    • MAX-interacting protein 2 antibody
    • Mitogen Activated Protein Kinase 14 antibody
    • Mitogen activated protein kinase p38 alpha antibody
    • Mitogen-activated protein kinase 14 antibody
    • Mitogen-activated protein kinase p38 alpha antibody
    • MK14_HUMAN antibody
    • Mxi 2 antibody
    • MXI2 antibody
    • p38 ALPHA antibody
    • p38 antibody
    • p38 MAP kinase antibody
    • p38 MAPK antibody
    • p38 mitogen activated protein kinase antibody
    • p38ALPHA antibody
    • p38alpha Exip antibody
    • PRKM14 antibody
    • PRKM15 antibody
    • RK antibody
    • SAPK2A antibody
    see all

Anti-p38 (phospho T180 + Y182) antibody images

  • All lanes : Anti-p38 (phospho T180 + Y182) antibody (ab4822) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) exposed for 40 min with UV, cell lysate
    Lane 3 : A431 (human epidermoid carcinoma cell line) cell lysate
    Lane 4 : A431 (human epidermoid carcinoma cell line) exposed for 40 min with UV, cell lysate
    Lane 5 : COLO 205 (human colon adenocarcinoma cell line) cell lysate
    Lane 6 : COLO 205 (human colon adenocarcinoma cell line) exposed for 40 min with UV, cell lysate
    Lane 7 : A549 (human lung carcinoma cell line) cell lysate
    Lane 8 : A549 (human lung carcinoma cell line) exposed for 40 min with UV, cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat anti-Rabbit IgG HRP at 1/5000 dilution
    Developed using the ECL technique

    Predicted band size : 38 kDa
  • Paraffin-embedded human brain tissue stained for p38 (phospho T180 + Y182) using ab4822 (right panel) at 1/100 dilution in immunohistochemical analysis followed by HRP-conjugated secondary antibody and DAB staining. Negative control (left panel) staining without primary antibody. 

  • Paraffin-embedded human heart tissue stained for p38 (phospho T180 + Y182) using ab4822 (right panel) at 1/20 dilution in immunohistochemical analysis followed by HRP-conjugated secondary antibody and DAB staining. Negative control (left panel) staining without primary antibody.

  • Paraffin-embedded rat heart tissue stained for p38 (phospho T180 + Y182) using ab4822 (right panel) at 1/20 dilution in immunohistochemical analysis followed by HRP-conjugated secondary antibody and DAB staining. Negative control (left panel) staining without primary antibody.

  •  


  • Predicted band size : 38 kDa
    Peptide Competition: Extracts prepared from HEK 293 cells treated with UV irradiation were resolved on a 10% Tris-glycine gel and transferred to nitrocellulose. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4oC, then were incubated with 0.50 µg/mL ab4822 for two hours at room temperature in a 3% BSA-TBST buffer, following its prior incubation with: the peptide immunogen (1), a generic phosphothreonine containing peptide (2), a generic phosphotyrosine-containing peptide (3), the non-phosphorylated peptide corresponding to the phosphopeptide (4), no peptide (5), the phosphorylated peptide derived from the corresponding region of JNK 1 & 2 (6), and, the phosphorylated peptide derived from the corresponding region of ERK 1 & 2 (7). After washing, membranes were incubated with goat F(ab')2 antirabbit IgG alkaline phosphatase and the signal was detected using the Tropix WesternStar method. The data show that only the phosphopeptide

References for Anti-p38 (phospho T180 + Y182) antibody (ab4822)

This product has been referenced in:
  • Kuanpradit C  et al. Attenuation of UV-B exposure-induced inflammation by abalone hypobranchial gland and gill extracts. Int J Mol Med 39:1083-1090 (2017). WB ; Human . Read more (PubMed: 28358420) »
  • Qiao Y  et al. Resveratrol ameliorates diabetic nephropathy in rats through negative regulation of the p38 MAPK/TGF-ß1 pathway. Exp Ther Med 13:3223-3230 (2017). Read more (PubMed: 28588674) »

See all 18 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Dog Cell lysate - whole cell (MDCK)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
10 µg
Treatment
100 pM IL-1β
Specification
MDCK
Blocking step
BlockAce as blocking agent for 50 minute(s) · Concentration: 100% · Temperature: 25°C
Username

令 中野

Verified customer

Submitted Jul 14 2017

Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
Sample
Mouse Tissue sections (retina)
Specification
retina
Permeabilization
Yes - triton x100
Fixative
Paraformaldehyde
Username

Dr. Minzhong Yu

Verified customer

Submitted Feb 27 2015

Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have read the details you have kindly provided and have following further questions for better understanding of the problem;

S...

Read More

Thank you for contacting us. I am sorry to hear the customer has been having problems with this antibody. I think the right band is being detected at ~55 kDa. I would like to suggest some tips that might help improve the results: - Decr...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Human Caco-2 epithelial colorectal adenocarcinoma)
Loading amount
10 µg
Specification
Human Caco-2 epithelial colorectal adenocarcinoma
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jan 06 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Tissue lysate - whole (skeletal muscle)
Loading amount
20 µg
Specification
skeletal muscle
Gel Running Conditions
Reduced Denaturing (Bis-Tris 4-12% MOPS running buffer)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Feb 25 2008

Thank you for your enquiry and patience. The concentration for this batch is 0.125 mg/ml. If you have any additional questions, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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