Recombinant
RabMAb

Anti-p38 (phospho T180 + Y182) antibody [EPR18120] (ab195049)

Overview

  • Product name
    Anti-p38 (phospho T180 + Y182) antibody [EPR18120]
    See all p38 primary antibodies
  • Description
    Rabbit monoclonal [EPR18120] to p38 (phospho T180 + Y182)
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Dot blotmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human p38 aa 150-250 (phospho T180 + Y182). The exact sequence is proprietary.
    Database link: Q16539

  • Positive control
    • WB: HEK-293 whole cell lysate treated with UV (60 J/M2) for 60 minutes; NIH/3T3 whole cell lysate treated with UV (60 J/M2) for 60 minutes.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab195049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
Dot blot 1/1000.

Target

  • Function
    Responds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.
  • Tissue specificity
    Brain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.
  • Sequence similarities
    Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Domain
    The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications
    Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CSAID Binding Protein 1 antibody
    • CSAID binding protein antibody
    • CSAID-binding protein antibody
    • Csaids binding protein antibody
    • CSBP 1 antibody
    • CSBP 2 antibody
    • CSBP antibody
    • CSBP1 antibody
    • CSBP2 antibody
    • CSPB1 antibody
    • Cytokine suppressive anti-inflammatory drug-binding protein antibody
    • EXIP antibody
    • MAP kinase 14 antibody
    • MAP kinase MXI2 antibody
    • MAP kinase p38 alpha antibody
    • MAPK 14 antibody
    • MAPK14 antibody
    • MAX interacting protein 2 antibody
    • MAX-interacting protein 2 antibody
    • Mitogen Activated Protein Kinase 14 antibody
    • Mitogen activated protein kinase p38 alpha antibody
    • Mitogen-activated protein kinase 14 antibody
    • Mitogen-activated protein kinase p38 alpha antibody
    • MK14_HUMAN antibody
    • Mxi 2 antibody
    • MXI2 antibody
    • p38 ALPHA antibody
    • p38 antibody
    • p38 MAP kinase antibody
    • p38 MAPK antibody
    • p38 mitogen activated protein kinase antibody
    • p38ALPHA antibody
    • p38alpha Exip antibody
    • PRKM14 antibody
    • PRKM15 antibody
    • RK antibody
    • SAPK2A antibody
    see all

Images

  • Dot blot analysis of p38 (phospho T180) peptide (Lane 1), p38 (phospho Y182) (Lane 2), p38 (phospho T180 + Y182) peptide (Lane 3), and non-phospho peptide (Lane 4), labeled using ab195049 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody (ab97051) at 1/1000 dilution.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • All lanes : Anti-p38 (phospho T180 + Y182) antibody [EPR18120] (ab195049) at 1/1000 dilution

    Lane 1 : Untreated HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysates
    Lane 2 : HEK-293 whole cell lysates treated with UV (60 J/M2) for 60 minutes

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

    Predicted band size: 41 kDa
    Observed band size: 41 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-p38 (phospho T180 + Y182) antibody [EPR18120] (ab195049) at 1/1000 dilution

    Lane 1 : Untreated NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate
    Lane 2 : NIH/3T3 whole cell lysate treated with UV (60 J/M2) for 60 minutes

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution

    Predicted band size: 41 kDa
    Observed band size: 41 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

References

This product has been referenced in:
  • Altshuler A  et al. RAS Regulates the Transition from Naive to Primed Pluripotent Stem Cells. Stem Cell Reports 10:1088-1101 (2018). WB ; Mouse . Read more (PubMed: 29456180) »
  • Thombare K  et al. Long chain saturated and unsaturated fatty acids exert opposing effects on viability and function of GLP-1-producing cells: Mechanisms of lipotoxicity. PLoS One 12:e0177605 (2017). WB . Read more (PubMed: 28520810) »

See all 8 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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