Anti-p38 (phospho T180 + Y182) antibody [M139] (ab45381)

Overview

  • Product nameAnti-p38 (phospho T180 + Y182) antibody [M139]
    See all p38 primary antibodies
  • Description
    Mouse monoclonal [M139] to p38 (phospho T180 + Y182)
  • SpecificityThis antibody detects a 42 kDa protein corresponding to the apparent molecular mass of p38a. This peptide sequence is highly conserved in the p38ß,g, and d MAPKs, and is identical in human and mouse p38a.
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Rat p38 (phospho T180 + Y182) conjugated to Keyhole Limpet Haemocyanin (KLH). Clone M139 was generated from a phospho-p38α (Thr-180/Tyr-182) synthetic peptide (coupled to KLH) corresponding to amino acid residues around threonine 180 and tyrosine 182 of rat p38α.

  • Positive control
    • Pervanadate treated human Jurkat and A431 cells and anisomycin treated human HeLa cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium azide
    Constituents: 50% PBS, 50% Glycerol, 0.1% BSA
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesMouse monoclonal purified with protein A chromatography.
  • ClonalityMonoclonal
  • Clone numberM139
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab45381 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 42 kDa.
ELISA 1/2000.

Peptide ELISA only.

ICC/IF 1/250.

Target

  • FunctionResponds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.
  • Tissue specificityBrain, heart, placenta, pancreas and skeletal muscle. Expressed to a lesser extent in lung, liver and kidney.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    Contains 1 protein kinase domain.
  • DomainThe TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
  • Post-translational
    modifications
    Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • CSAID Binding Protein 1 antibody
    • CSAID binding protein antibody
    • CSAID-binding protein antibody
    • Csaids binding protein antibody
    • CSBP 1 antibody
    • CSBP 2 antibody
    • CSBP antibody
    • CSBP1 antibody
    • CSBP2 antibody
    • CSPB1 antibody
    • Cytokine suppressive anti-inflammatory drug-binding protein antibody
    • EXIP antibody
    • MAP kinase 14 antibody
    • MAP kinase MXI2 antibody
    • MAP kinase p38 alpha antibody
    • MAPK 14 antibody
    • MAPK14 antibody
    • MAX interacting protein 2 antibody
    • MAX-interacting protein 2 antibody
    • Mitogen Activated Protein Kinase 14 antibody
    • Mitogen activated protein kinase p38 alpha antibody
    • Mitogen-activated protein kinase 14 antibody
    • Mitogen-activated protein kinase p38 alpha antibody
    • MK14_HUMAN antibody
    • Mxi 2 antibody
    • MXI2 antibody
    • p38 ALPHA antibody
    • p38 antibody
    • p38 MAP kinase antibody
    • p38 MAPK antibody
    • p38 mitogen activated protein kinase antibody
    • p38ALPHA antibody
    • p38alpha Exip antibody
    • PRKM14 antibody
    • PRKM15 antibody
    • RK antibody
    • SAPK2A antibody
    see all

Anti-p38 (phospho T180 + Y182) antibody [M139] images


  • Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 42 kDa
    Observed band size : 42 kDa


    Exposure time : 2 minutes

    Serum starved HepG2 cells were incubated at 37°C for 30 minutes with vehicle control (0 μM) and different concentrations of (±)-naringenin (ab120958). Increased expression of p38 (phospho T180 + Y182) (

    Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10μg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with μg /ml and ab8227 at 1/1000 dilution overnight at 4°C. Antibody binding was detected using an anti-mouse antibody conjugated to HRP (ab97040) at 1/10000 and visualised using ECL development solution.

  • All lanes : Anti-p38 (phospho T180 + Y182) antibody [M139] (ab45381) at 1/1000 dilution

    Lane 1 : A431 cells serum starved overnight
    Lane 2 : A431 cells treated with pervanadate (1 mM) for 30 minutes
    Lane 3 : A431 cells treated with pervanadate (1 mM) for 30 minutes with No peptide
    Lane 4 : A431 cells treated with pervanadate (1 mM) for 30 minutes with phospho-ERK1 (T202/Y204) peptide
    Lane 5 : A431 cells treated with pervanadate (1 mM) for 30 minutes with phoshpo-p38 (T180/Y182) peptide

    Lysates/proteins at 20 µg per lane.


    Predicted band size : 42 kDa
    Observed band size : 42 kDa
  • Immunofluorescence analysis of pervanadate treated mouse C2C12 cells, staining p38 (phospho T180 + Y182) with ab45381 at 1/250 dilution. Cy3-conjugated anti-mouse IgG was used as the secondary antibody.

References for Anti-p38 (phospho T180 + Y182) antibody [M139] (ab45381)

This product has been referenced in:
  • Li X  et al. The selective NADPH oxidase inhibitor apocynin has potential prophylactic effects on melamine-related nephrolithiasis in vitro and in vivo. Mol Cell Biochem 399:167-78 (2015). Read more (PubMed: 25318609) »
  • Naviglio S  et al. Leptin potentiates antiproliferative action of cAMP elevation via protein kinase A down-regulation in breast cancer cells. J Cell Physiol 225:801-9 (2010). WB ; Human . Read more (PubMed: 20589829) »

See all 3 Publications for this product

Product Wall

Application Western blot
Sample Rat Tissue lysate - whole (muscle)
Gel Running Conditions Reduced Denaturing (9)
Loading amount 40 µg
Specification muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Submitted Nov 01 2016



As we discussed over the phone, I requested further information as to the protocol used to produce the Western blot presented on the datasheet of ab31828 and ab45381. I have attached the protocol to this email. In summary, the pervandate trea...

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