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Anti-p40 antibody (ab12109)

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Overview

Product name

Anti-p40 antibody
See all p40 products (5) ...

Description

Rabbit polyclonal to p40

Tested applications

WBmore details

Cross reactivity

Reacts with

Mouse, Human

Predicted to work with

Xenopus laevis

Immunogen

Synthetic peptide derived from residues 200 to the C-terminus of Human p40.

Positive control

Endogenous protein detectable in Hela cells by Western Blot.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

  • Western blot - p40 antibody (ab12109)Western blot - p40 antibody (ab12109) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab12109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ICC/IF: Use at a concentration of 5 µg/ml.
WB: Use at a concentration of 0.2 (endogenous) - 1 (recombinant) µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 28 kDa).

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Rab9 effector required for endosome to trans-Golgi network (TGN) transport.

Sequence similarities

Contains 5 Kelch repeats.

Post-translational
modifications

Phosphorylated on Ser residues most probably by PIP5K3.

Cellular localization

Cytoplasm. Endosome membrane. Interaction with PIP5K3 and subsequent phosphorylation recruits it to the endosomal membrane.

Target information above from: UniProt accessionQ7Z6M1 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • 8430412M01Rik antibody
  • AV073337 antibody
  • C87311 antibody
  • 40 kDa Rab9 effector protein antibody
  • 9530020d24rik antibody
  • bA65N13.1 antibody
  • DKFZp686P1077 antibody
  • OTTHUMP00000022126 antibody
  • OTTMUSP00000012898 antibody
  • OTTMUSP00000012899 antibody
  • OTTMUSP00000041318 antibody
  • p40 antibody
  • RAB9 effector p40 antibody
  • Rab9 effector protein with Kelch motifs antibody
  • RAB9p40 antibody
  • RABEK_HUMAN antibody
  • RABEPK antibody
  • RABEPK protein antibody
  • RGD1310612 antibody
  • RP11 65N13.1 antibody
  • RP23-446N16.2 antibody
see all

Anti-p40 antibody images:

  Western blot - p40 antibody (ab12109)

Western blot - p40 antibody (ab12109)



Performed under reducing conditions.

Predicted band size : 28 kDa
Additional bands at : 32 kDa (possible cross reactivity),47 kDa (possible cross reactivity),49 kDa (possible cross reactivity),60 kDa (possible cross reactivity).

ab12109 recognises p40 in Western Blots. A: 293 HEK cells were transfected with negative control cDNA (pCDNA3 vector) or human p40 cDNA. B: negative control cDNA (pCDNA3 vector) or human p40 cDNA were in vitro translated in rabbit reticulocyte lysate. Blots were subjected to ab12109 diluted 1/2500. Exposure was for 1 minute. A band corresponding to the expected MW of p40 was detected (arrow) in all lanes except negative control lanes. Exposure time: 1 min

References for Anti-p40 antibody (ab12109)

ab12109 has not yet been referenced specifically in any publications.

Publishing research using ab12109? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"