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Western blot - p40 antibody (ab12109)
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Immunocytochemistry/ Immunofluorescence - p40 antibody (ab12109)
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Product Name
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p40 antibody
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See all p40 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to p40
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Immunogen
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Synthetic peptide derived from residues 200 to the C-terminus of Human p40.
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Reacts with
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Hu, Ms
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Predicted to react
(species key)
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Xl
(Due to sequence homology)
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Tested applications
(see key)
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ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 5 µg/ml.
WB: Use at a concentration of 0.2 (endogenous) - 1 (recombinant) µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 28 kDa).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Endogenous protein detectable in Hela cells by Western Blot.
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Cellular localization
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Endosomial
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Research areas
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Cell Biology >> Other Antibodies >> Other Antibodies
Signal Transduction >> Protein Trafficking >> Golgi Proteins
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Relevance
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RAB GTPases are involved in the docking of transport vesicles with their cognate target membranes. The association of RAB proteins with membranes is enabled by their doubly geranylgeranylated, hydrophobic C termini. RAB9 GTPase is required for the stimulation of mannose 6-phosphate receptor transport from endosomes to the trans-Golgi network. p40 is thought to be a RAB9 effector protein with kelch motifs.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this p40 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for p40 antibody images, references, products related to ab12109 and other tools.
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p40 antibody images:
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 Western blot - p40 antibody (ab12109)
Detection of recombinant human p40 using ab12109
Performed under reducing conditions.
Predicted band size : 28 kDa
Additional bands at : 32 kDa (possible cross reactivity),47 kDa (possible cross reactivity),49 kDa (possible cross reactivity),60 kDa (possible cross reactivity).
ab12109 recognises p40 in Western Blots.
A: 293 HEK cells were transfected with negative control cDNA (pCDNA3 vector) or human p40 cDNA.
B: negative control cDNA (pCDNA3 vector) or human p40 cDNA were in vitro translated in rabbit reticulocyte lysate.
Blots were subjected to ab12109 diluted 1/2500. Exposure was for 1 minute.
A band corresponding to the expected MW of p40 was detected (arrow) in all lanes except negative control lanes. Exposure time: 1 min
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Immunocytochemistry/ Immunofluorescence - p40 antibody (ab12109)
ICC/IF image of ab12109 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab12109, 5µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
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Search PubMed (MEDLINE) for references to p40
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p40 antibody - more information
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Customer reviews (feedback) regarding p40 antibody |
Customer FAQs regarding p40 antibody |
Protocols for p40 antibody |
Price and availability of products related to p40 antibody |
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p40 antibody - related products:
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p40 antibody - other tools:
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Contact Abcam with a Technical Enquiry about p40 antibody |
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